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Genetics & The Work of Mendel

Genetics & The Work of Mendel. What is Genetics?. Genetics studies heredity. Heredity is the passing of traits from parents to offspring. Who is Father of Genetics?.

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Genetics & The Work of Mendel

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  1. Genetics &The Work of Mendel

  2. What is Genetics? • Genetics studies heredity. • Heredity is the passing of traits from parents to offspring.

  3. Who is Father of Genetics? • Gregor Mendel – in mid-1800s, Austrian monk who was the first person to succeed in predicting how traits passed from parent to offspring • He used garden peas in his experiments.

  4. Mendel’s Experiments • He controlled his experiments to ensure accurate results: • Self-Pollination – pea plant would pollinate itself • Cross-Pollination – one pea plant would pollinate another

  5. Mendel’s Monohybrid Crosses • Mendel selected a white-flower plant and a purple-flower plant. • He crossed them to produce new plants. • Hybrid – offspring of parents that have different forms of a trait (ex. Tall and short height) • Monohybrid – “one trait”

  6. Vocabulary 1. Gene – portion of DNA that codes for a trait or protein 2. Trait – inheritable characteristic 3. Allele – number of alternative forms of the same gene or same genetic locus (spot)

  7. Vocabulary 4. Dominant allele/trait – trumps other alleles (hides them) and is written with an uppercase letter ex. Brown eyes are dominant = B 5. Recessive allele/trait – hidden if dominant allele is present and is written with a lowercase letter ex. Blue eyes are recessive = b

  8. Vocabulary 6. Homozygous – both forms of the allele are the same; also known as purebred, true-breeding ex. Homozygous brown eyes = BB Homozygous blue eyes = bb 7. Heterozygous – forms of the allele are different; also known as hybrid ex. Heterozygous brown eyes = Bb

  9. Mendel’s Work Pollen transferred from white flower to stigma of purple flower P • Parent generation (P): • White plant x Purple plant • First generation (F1 ): • Produced all purple plants • F = filial • Second generation (F2): • Self pollinate F1generation • Purple plant x purple plant produced 3 purple plants & 1 white plant. anthers removed all purple flowers result F1 self-pollinate F2

  10. true-breeding purple-flower peas true-breeding white-flower peas 100% purple-flower peas 100% F1 generation (hybrids) 75% purple-flower peas 25% white-flower peas 3:1 F2 generation Looking closer at Mendel’s work X P self-pollinate

  11. What did Mendel’s findings mean? • Traits come in alternative versions: • Purple vs. White flower color • Alleles- a number of alternative forms of the same gene or same genetic locus (spot) • some difference in sequence of A, T, C, G purple-flower allele & white-flower allele are two DNA variations at flower-color locus different versions of gene at same location on homologous chromosomes

  12. What did Mendel’s findings mean? • Some traits mask others: • purple & white flower colors are separate traits that do not blend • purple x white ≠ light purple • purplemaskedwhite • Dominant allele = purple flower color • Recessive allele = white flower color White Allele Recessive Trait Purple Allele Dominant Trait homologouschromosomes

  13. X P purple white F1 all purple Genotype vs. Phenotype • Difference between how an organism “looks” & its genetics: 1. Phenotype - description of organism’s trait • Phenotype = Physical 2. Genotype - description of organism’s genetic makeup • Genotype = Genetics Explain Mendel’s results using …dominant&recessive …phenotype&genotype

  14. ff FF x X P purple white F1 all purple Making Crosses • Can represent alleles as letters: **when choosing letters, pick letter where uppercase looks different then lowercase • flower color alleles  F or f • true-breeding purple-flower peas  FF • true-breeding white-flower peas  ff F F f Ff Ff f Ff Ff

  15. true-breeding purple-flower peas true-breeding white-flower peas 100% purple-flower peas F1 generation (hybrids) 100% 75% purple-flower peas 25% white-flower peas Looking closer at Mendel’s work X P phenotype ff FF genotype Ff Ff Ff Ff self-pollinate 3:1 F2 generation What are genotypes of F2? ? ? ? ?

  16. male / sperm F f F female / eggs f Punnett Squares Aaaaah, phenotype & genotypecan have different ratios!! Ff x Ff F1 generation (hybrids) Phenotype Genotype FF = 25% Ff = 50% ff = 25% Purple = 75% White = 25% Ff FF 3:1 ratio Ff ff 1:2:1 ratio

  17. T T Tt Tt Tt Tt t t Mendel’s Laws of Heredity • Law of Segregation: • Alleles for SAME trait separate into different gametes during meiosis. • Ex. Height • Law of Independent Assortment: • Alleles for DIFFERENT traits separate independently of each other during meiosis. • Ex. Blonde hair does not mean you have blue eyes. Traits inherited independently.

  18. Monohybrid Crosses Practice • Every genetics problem you work must include: • Key • Cross (Parent x Parent) • Punnett Square • Results (Genotype & Phenotype)

  19. Monohybrid Crosses Practice 1. Black-colored (B) bear is dominant over brown-colored (b) bear. Cross a homozygous dominant bear with a heterozygous bear. Give phenotypic and genotypic results of F1 generation. B Key B = black b = brown Cross: BB x Bb B Genotype BB = 50% Bb = 50% 1:1 Ratio Phenotype Black = 100% B BB BB b Bb Bb

  20. Monohybrid Crosses Practice 2. In human, dimples (D) is dominant over no dimples (d). Cross a hybrid woman with a man who does not have dimples. Give phenotypic and genotypic results of F1 generation. What are the chances the couple will have a child with no dimples? Key D = dimples d = no dimples Cross: Dd x dd d D Genotype Dd = 50% dd = 50% 1:1 Ratio Phenotype Dimples = 50% No dimples = 50% 1:1 Ratio d Dd dd d dd Dd 50% chance of child with no dimples

  21. 3. Curly hair (H) is dominant over straight hair (h). Cross a purebred curly haired woman with a true-breeding straight haired man. Give phenotypic and genotypic results of F1 generation. Then, cross F1 offspring to show phenotypic and genotypic results of F2 generation. H H Key H = curly h = straight Cross: HH x hh Genotype Hh = 100% Phenotype Curly = 100% h Hh Hh F1 h Hh Hh h H Key H = curly h = straight Cross: Hh x Hh Genotype HH = 25% Hh = 50% hh = 25% 1:2:1 Ratio Phenotype Curly = 75% Straight = 25% 3:1 Ratio H HH Hh F2 h hh Hh

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