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Oxydo-reducteur systems and the thiol-dependent antioxidant equipment in Melampsora

Oxydo-reducteur systems and the thiol-dependent antioxidant equipment in Melampsora. Benjamin Selles, Nicolas Rouhier, Eric Gelhaye. Oxydo-reducteur systems and the thiol-dependent antioxidant equipment in Melampsora. Oxygen is essential, but highly toxic.

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Oxydo-reducteur systems and the thiol-dependent antioxidant equipment in Melampsora

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  1. Oxydo-reducteur systems and the thiol-dependent antioxidant equipment in Melampsora Benjamin Selles, Nicolas Rouhier, Eric Gelhaye

  2. Oxydo-reducteur systems and the thiol-dependent antioxidant equipment in Melampsora Oxygen is essential, but highly toxic Formation of reactive oxygen species (ROS) due to electron transport chains and oxidase functioning Diffusion within cells : importance to have an antioxidant equipment in all sub-cellular compartments Release of ROS by plants as defense mechanism against pathogens

  3. ROS formation

  4. 2 H+ + 2 e- 02.- + 02.- H2O2 + 02 H2O2 + H2O2 2H2O + O2 H2O ROOH + RSH2 ROH + RS2 + H2O MetSO + RSH2 Met + RS2 + Superoxide and peroxide scavenging • Superoxide dismutases • Catalases : haem peroxidase (peroxisomes) • Thiol peroxidases : haem free thiol dependent peroxidases • peroxiredoxins & glutathione peroxidases • some specificglutathione-S-transferases • Methionine sulfoxide reductases : • indirect massive detoxification through MetSO reduction H2O2

  5. SOD, catalases, thiol-peroxidases, methionine sulfoxide reductases

  6. SOD, catalases, thiol-peroxidases, methionine sulfoxide reductases

  7. 1-cysPrx Thiol peroxidase phylogenetic tree 2-cysPrx Mlp1-cys MlpPrxII PrxII MlpPrxQ PrxQ MlpGpx Gpx

  8. Methionine sulfoxide reductasesphylogenetic tree MlpMsrB MlpMsrA

  9. Glutathione-S-transferases • Complex and widespread superfamily • Share thioredoxin fold • Prominent roles : • phase II detoxication enzymes • Glutathione peroxidase • Nitrogen metabolism… • Poorly characterized in fungi

  10. Cluster3/Omega-like A. nidulans (AN10695) L. bicolor (LACBI1231002) C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549) C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218) P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880) 2 P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790) A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683) A. nidulans (AN4905) N. crassa (NCU04109) Cluster2 A. nidulans (AN9299) C. cinereus (CC1G00247.1) C. cinereus (CC1G00666.1) 4 L. bicolor (LACBI1188517) P. chrysosporium (Phchr503) Ure2p A. nidulans (AN3255) N. crassa (NCU05780) P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271) P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511) S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1) L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) GTT2 L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971) L. bicolor (LACBI1240633) L. bicolor (LACBI1314362) S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595) 1 A. nidulans (AN6563) N. crassa (NCU03826) S. cerevisiae (YKL081W) EF1B S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C) A. nidulans (AN9304) 1 N. crassa (NCU10521.3) A. nidulans (AN0629) N. crassa (NCU05706) Cluster1/GTT1 C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C) A. nidulans (AN10273) N. crassa (NCU04368) Omega C. cinereus (CC1G04116.1) 1 C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379) A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) Etherases C. cinereus (CC1G12612.1) C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118) P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1) C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1) C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1) C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115) P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) MAK16 S. cerevisiae (YAL025C) C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531) N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850) P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857) Figure 1 0.1

  11. Cluster3/Omega-like A. nidulans (AN10695) L. bicolor (LACBI1231002) C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549) C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218) P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880) 2 P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790) A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683) A. nidulans (AN4905) N. crassa (NCU04109) 4 : oxidative stress, Glutathione peroxidase, nitrogen metabolism regulation Cluster2 A. nidulans (AN9299) C. cinereus (CC1G00247.1) C. cinereus (CC1G00666.1) L. bicolor (LACBI1188517) P. chrysosporium (Phchr503) Ure2p A. nidulans (AN3255) N. crassa (NCU05780) P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271) P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511) S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1) L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) GTT2 L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971) L. bicolor (LACBI1240633) L. bicolor (LACBI1314362) S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595) 1 A. nidulans (AN6563) N. crassa (NCU03826) S. cerevisiae (YKL081W) EF1B S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C) A. nidulans (AN9304) 1 N. crassa (NCU10521.3) A. nidulans (AN0629) N. crassa (NCU05706) Cluster1/GTT1 C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C) A. nidulans (AN10273) N. crassa (NCU04368) Omega C. cinereus (CC1G04116.1) 1 C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379) A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) Etherases C. cinereus (CC1G12612.1) C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118) P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1) C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1) C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1) C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115) P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) MAK16 S. cerevisiae (YAL025C) C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531) N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850) P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857) Figure 1 0.1

  12. Cluster3/Omega-like A. nidulans (AN10695) L. bicolor (LACBI1231002) C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549) C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218) P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880) 2 P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790) A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683) A. nidulans (AN4905) N. crassa (NCU04109) Cluster2 A. nidulans (AN9299) C. cinereus (CC1G00247.1) C. cinereus (CC1G00666.1) 4 L. bicolor (LACBI1188517) P. chrysosporium (Phchr503) Ure2p A. nidulans (AN3255) N. crassa (NCU05780) P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271) P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511) S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1) L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) GTT2 L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971) L. bicolor (LACBI1240633) L. bicolor (LACBI1314362) S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595) 1: Elongation factor A. nidulans (AN6563) N. crassa (NCU03826) S. cerevisiae (YKL081W) EF1B S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C) A. nidulans (AN9304) 1 N. crassa (NCU10521.3) A. nidulans (AN0629) N. crassa (NCU05706) Cluster1/GTT1 C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C) A. nidulans (AN10273) N. crassa (NCU04368) Omega C. cinereus (CC1G04116.1) 1 C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379) A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) Etherases C. cinereus (CC1G12612.1) C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118) P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1) C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1) C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1) C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115) P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) MAK16 S. cerevisiae (YAL025C) C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531) N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850) P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857) Figure 1 0.1

  13. Cluster3/Omega-like A. nidulans (AN10695) L. bicolor (LACBI1231002) C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549) C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218) P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880) 2 P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790) A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683) A. nidulans (AN4905) N. crassa (NCU04109) Cluster2 A. nidulans (AN9299) C. cinereus (CC1G00247.1) C. cinereus (CC1G00666.1) 4 L. bicolor (LACBI1188517) P. chrysosporium (Phchr503) Ure2p A. nidulans (AN3255) N. crassa (NCU05780) P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271) P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511) S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1) L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) GTT2 L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971) L. bicolor (LACBI1240633) L. bicolor (LACBI1314362) S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595) 1 A. nidulans (AN6563) N. crassa (NCU03826) S. cerevisiae (YKL081W) EF1B S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C) A. nidulans (AN9304) 1: Glutathione peroxidase N. crassa (NCU10521.3) A. nidulans (AN0629) N. crassa (NCU05706) Cluster1/GTT1 C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C) A. nidulans (AN10273) N. crassa (NCU04368) Omega C. cinereus (CC1G04116.1) 1 C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379) A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) Etherases C. cinereus (CC1G12612.1) C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118) P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1) C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1) C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1) C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115) P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) MAK16 S. cerevisiae (YAL025C) C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531) N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850) P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857) Figure 1 0.1

  14. Cluster3/Omega-like A. nidulans (AN10695) L. bicolor (LACBI1231002) C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549) C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218) P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880) 2 P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790) A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683) A. nidulans (AN4905) N. crassa (NCU04109) Cluster2 A. nidulans (AN9299) C. cinereus (CC1G00247.1) C. cinereus (CC1G00666.1) 4 L. bicolor (LACBI1188517) P. chrysosporium (Phchr503) Ure2p A. nidulans (AN3255) N. crassa (NCU05780) P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271) P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511) S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1) L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) GTT2 L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971) L. bicolor (LACBI1240633) L. bicolor (LACBI1314362) S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595) 1 A. nidulans (AN6563) N. crassa (NCU03826) S. cerevisiae (YKL081W) EF1B S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C) A. nidulans (AN9304) 1 N. crassa (NCU10521.3) A. nidulans (AN0629) N. crassa (NCU05706) Cluster1/GTT1 C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C) A. nidulans (AN10273) N. crassa (NCU04368) Omega C. cinereus (CC1G04116.1) 1: Dihydroascorbate reductase, thiol transferase C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379) A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) Etherases C. cinereus (CC1G12612.1) C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118) P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1) C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1) C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1) C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115) P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) MAK16 S. cerevisiae (YAL025C) C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531) N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850) P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857) Figure 1 0.1

  15. Cluster3/Omega-like A. nidulans (AN10695) L. bicolor (LACBI1231002) C. cinereus (CC1G06890.1) P. chysosporium (EU791893) P. chysosporium (Phchr7168) P. chysosporium (Phchr7169) N. crassa (NCU00549) C. cinereus (CC1G10591.1) L. bicolor (LACBI1299218) P. chrysosporium (Phchr3911) P. chrysosporium (Phchr6880) 2 ? P. chrysosporium (Phchr6881) L. bicolor (LACBI1330814) N. crassa (NCU01347.3) L. bicolor (LACBI1189790) A. nidulans (AN3299) P. chrysosporium (Phchr6766) C. cinereus (CC1G12668.1) L. bicolor (LACBI1300632) P. chrysosporium (Phchr6683) A. nidulans (AN4905) N. crassa (NCU04109) Cluster2 A. nidulans (AN9299) C. cinereus (CC1G00247.1) C. cinereus (CC1G00666.1) 4 L. bicolor (LACBI1188517) P. chrysosporium (Phchr503) Ure2p A. nidulans (AN3255) N. crassa (NCU05780) P. chrysosporium (Phchr140156) P. chrysosporium (Phchr137250) P. chrysosporium (Phchr2269) P. chrysosporium (Phchr2266) P. chrysosporium (Phchr140259) P. chrysosporium (Phchr140271) P. chrysosporium (Phchr2268) P. chrysosporium (Phchr128511) S. cerevisiae (YNL229C) C. cinereus (CC1G10742.1) C. cinereus (CC1G10750.1) L. bicolor (LACBI1184665) L. bicolor (LACBI1296671) GTT2 L. bicolor (LACBI1298606) C. cinereus (CC1G11890.1) C. cinereus (CC1G12718.1) P. chrysosporium (Phchr7971) L. bicolor (LACBI1240633) L. bicolor (LACBI1314362) S. cerevisiae (YLL060C) A. nidulans (AN10038) A. nidulans (AN1595) 1 A. nidulans (AN6563) N. crassa (NCU03826) S. cerevisiae (YKL081W) EF1B S. cerevisiae (YPL048W) C. cinereus (CC1G01604.1) L. bicolor (LACBI1248920) P. chrysosporium (Phchr39727) S. cerevisiae (YGR201C) A. nidulans (AN9304) 1 N. crassa (NCU10521.3) A. nidulans (AN0629) N. crassa (NCU05706) Cluster1/GTT1 C. cinereus (CC1G13373.1) L. bicolor (LACBI1319137) S. cerevisiae (YIR038C) A. nidulans (AN10273) N. crassa (NCU04368) Omega C. cinereus (CC1G04116.1) 1 C. cinereus (CC1G11081.1) L. bicolor (LACBI1179508) P. chrysosporium (EU791894) P. chrysosporium (Phchr126388) S. cerevisiae (YGR154C) S. cerevisiae (YKR076W) S. cerevisiae (YMR251W) A. nidulans (AN5831) N. crassa (NCU09570) A. nidulans (AN10379) A. nidulans (AN2592) A. nidulans (AN2948) N. crassa (NCU02888) A. nidulans (AN8942) C. cinereus (CC1G00881.1) C. cinereus (CC1G01486.1) C. cinereus (CC1G04959.1) Etherases C. cinereus (CC1G12612.1) C. cinereus (CC1G05384.1) L. bicolor (LACBI1238438) L. bicolor (LACBI1295178) P. chrysosporium (Phchr5119) P. chrysosporium (Phchr5118) P. chrysosporium (Phchr5122) P. chrysosporium (Phchr5300) C. cinereus (CC1G02842.1) C. cinereus (CC1G05417.1) C. cinereus (CC1G07900.1) C. cinereus (CC1G07903.1) C. cinereus (CC1G07902.1) C. cinereus (CC1G07927.1) C. cinereus (CC1G07928.1) C. cinereus (CC1G02394.1) C. cinereus (CC1G07746.1) L. bicolor (LACBI1318115) P. chrysosporium (Phchr7058) L. bicolor (LACBI1311800) A. nidulans (AN6612) N. crassa (NCU04150) MAK16 S. cerevisiae (YAL025C) C. cinereus (CC1G05311.1) L. bicolor (LACBI1146679) P. chrysosporium (Phchr137531) N. crassa (NCU06035.3) S. cerevisiae (YAL003W) L. bicolor (LACBI1328850) P. chrysosporium (Phchr1288) L. bicolor (LACBI1245857) Figure 1 0.1

  16. Disulfide bond formation, reduction, isomerisation Regulation of protein folding, activity • Spontaneous (with oxygen) • Catalyzed by SOX • Concomitant to a reducing activity SH SOX SH S PDI Oxidation SH S S Reduction SH S SH TRX, GRX SH GRX CxxC/S redox centers SH SSG

  17. Msr ROS GPX PRX Grx Trx NTR GSH GR NADPH ROS S-SG Trx

  18. Disulfide bond reduction by glutaredoxins & thioredoxins

  19. MlpTrx4 MlpTrx1, 2, 3

  20. MlpGrx 4 MlpGrx 5 Fe/S cluster formation CGFS Trx/Grx domains CGFS MlpGrx 2.2 Dithiol CPYC MlpGrx1.1, 1.2 MlpGrx 2.1 CPYS

  21. Disulfide bond formation by sulfhydryl oxidases and isomerisation by protein disulfide isomerases TRX TRX Erv1 and TRX interaction in IMS; H2O2 production Erv1, TRX and CytC interaction in IMS; H2O production Oxidation and isomerization by QSOX (Erv2 for fungi) and PDI on unfolded proteins

  22. QSOX QSOX1 was initially identified from quiescent human fibroblasts and his Intracellular localisation is: ER, Golgi, secretory granules and are located at the cell surface Folding and regulation of secreted and parietal proteins SOX (ScErv1 like) and Erv2 First discovered in S cerevisiae and was essential for respiration and vegetative growth, Intracellular localisation is: IMS (SOX /scErv1 like) ER (Erv2, Fungi) Mitochondrial biogenesis Protein folding Cytosolic Fe-S protein maturation Protein folding PDI and PDI like First isolated from liver in 1963 Intracellular localisation is: ER Domaine TRX 1 Domaine TRX 2 Classe 1 Folding of proteins by isomerization of disulfide bonds N C CXXC CXXC Domaine TRX 1 Domaine TRX 2 Classe 2 N C CXXC CXXC Domaine TRX Classe 5 N C CXXC

  23. Comparative genomic with other sequenced fungi

  24. scErv2 like Basidiomycete Fungus Mellp (e_gw1.25.122.1) |Mellp (e_gw1.18.104.1) Mammalian Ascomycete Plants Plants Mammalian SOX (scErv1 like) QSOX (HsQuiecin like)

  25. PDI class 1 Basidiomycete Ascomycete Basidiomycete Asc estExt_fgenesh2_pg.C_450075 PDI class 5 EuGene.00190108 estExt_fgenesh2_pg.C_240082 Ascomycete Ascomycete EuGene.00020304 Basidiomycete Basidiomycete PDI class 2

  26. Conclusions Tpx PL-OOH PL ROS/RNS sensing and degradation H2O + 1/2O2 Sensing by TF H2O NO2 Cat Trx, Tpx Tpx, Grx, GST Tpx OH. H2O2 OONO- 02.- SOD Fe2+, Cu 2+ Fenton reaction NO. R(SH)2 RSSR, RSSR’, R(S)2 RSSG Grx, Srx? Trx, Grx RSH RSNO Trx, Grx Grx? Trx? GSH RSOH sulfenic Protein thiol oxidation Srx sulfonic RSO2H RSO3H sulfinic

  27. Conclusions Tpx PL-OOH PL ROS/RNS sensing and degradation H2O + 1/2O2 Sensing by TF H2O NO2 Cat Trx, Tpx Tpx, Grx, GST Tpx OH. H2O2 OONO- 02.- SOD Fe2+, Cu 2+ Fenton reaction NO. R(SH)2 RSSR, RSSR’, R(S)2 RSSG Grx, Srx? Trx, Grx RSH RSNO Trx, Grx Grx? Trx? GSH RSOH sulfenic Protein thiol oxidation Srx sulfonic RSO2H RSO3H sulfinic

  28. Heme- thiolate Haloperoxidases Hofrichter and Ulrich, 2006

  29. Pfam 1328: Peroxidase_2  Peroxidase, family 2. The peroxidases in this family do not have similarity to other peroxidases.

  30. Peroxidase class II (Peroxibase) Function ?? All predicted secreted (target P) Mlp Prx 12 isoforms

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