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Synthetic Biology at Davidson College. Building Bacterial Computers. Advantages of Biological Computers. go anywhere - arctic, thermal vents, inside organisms. no electricity. self-replicating. no immune rejection. Two Recent Research Projects. Flipping DNA with Hin/ hixC.

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Presentation Transcript
slide1

Synthetic Biology

at

Davidson College

Building Bacterial Computers

slide3

Advantages of Biological Computers

go anywhere - arctic, thermal vents, inside organisms

no electricity

self-replicating

no immune rejection

slide16

Engineering Biological HPP

Hin-mediated recombination

slide23

Split Genes to Encode Problem

gcat.davidson.edu/GcatWiki/index.php/Davidson_Missouri_W/Davidson_Protocols

slide24

Split Reporter Genes

Red Fluorescent Protein

Green Fluorescent Protein

slide25

Split Reporter Genes

GFP Split by hixC

RFP Split by hixC

slide29

Paper Published 7/09

Paper of the year, 2009

15 undergraduate coauthors

slide44

Redesign System v2.0

positive feedback loop

slide45

Outcomes of v 2.0

frame

shift

“leak”

+tRNA

CGGUC

+tRNA

CCACU

- control

+ control

slide46

Outcomes of v 2.0

frame

shift

“leak”

+tRNA

CGGUC

+tRNA

CCACU

+ control

slide50

Evolution of Bacterial Computers

E. coli in 2011

?

Living Hardware in 2021