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BIOL22332/20972 GENETICS / Dev.Biol RSM MODULE 3. The University of Manchester. Faculty of Life Sciences. Embedding, documentation & filing. Experimental background. What is lacZ?. The lac operon of E. coli. β -Gal expressing fly lines. X-lacZ. enhancer constructs.

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slide1

BIOL22332/20972

GENETICS / Dev.Biol RSM

MODULE 3

The University

of Manchester

Faculty of

Life Sciences

Embedding,

documentation

& filing

slide2

Experimental background

What is lacZ?

The lac operon of E. coli

slide5

β-Gal expressing fly lines

X-lacZ

enhancer constructs

X-Gal4 UAS-lacZ

X-lacZ

enhancer trap lines

slide6

gut

proventricle

head

skeleton

on epidermis:

wing, haltere, 3rd leg

on CNS:

1st & 2nd leg

slide8

Embedding of larval preparations

- transfer larval preparations to a drop of glycerol on a slide

- dissect leg and wing discs away and separate on one side of the drop

- place 40 μl droplet of glycerol on a fresh (frosted) slide

- transfer discs with one shank of the forceps (2-5 wing and 2-5 leg discs)

- put on cover slip (see image)

- seal all around the cover slip with nail varnish

slide11

Documentation

18.02.2005

John Smith

L3, genotype B

X-Gal

location: B28

arrow: wing margin

arrow head: wing hinge

slide12

Draw as many of the various genetic strains as possible to have material for the assessed task, i.e. composing a figure and its legend

slide13

set to '0'

'A' to 'B'

start small

focus of

condenser

use little contrast

'C' to 'D'

'B' to 'C'