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TANNINS AND HUMAN HEALTH: Nutraceuticals effect of Tannin and its Monomers. MAYANK TANDON & DR. S.N. RAI Animal Nutrition Division, National Dairy Research Institute, e-mail: mayanktandon1@gmail.com TANNINS

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slide1
TANNINS AND HUMAN HEALTH: Nutraceuticals effect of Tannin and its Monomers.

MAYANK TANDON & DR. S.N. RAI

Animal Nutrition Division,

National Dairy Research Institute,

e-mail: mayanktandon1@gmail.com

tannins
TANNINS
  • Plant Tannins: distinctive group of polyphenolic polymers of relatively high molecular weight (M.W = 1000 to 20,000)
  • Further categorized as: HYDROLYSABLE tannins (HTs) or CONDENSED tannins (CTs or Proanthocyandins) on the basis of their structure and reactivity.
slide3
HTs are of low M.W (500-3000) and are polyesters of Gallic acid (gallo tannins) and hexahydroxy-diphenic acid (ellagitannins) with a central polyols such as sugars/glucose and phenolic such as catechin.
  • CTs, M.W (1900-28000) have no carbohydrate core and comprise a group of polyhydroxy-flavan-3-ol oligomers and polymers linked by carbon-carbon bonds between flavanol subunits.
tannins basic structure
TANNINS (Basic structure)

Hydrolysable Tannins

Condensed Tannins

Glucose

o

oH

oH

oH

oH

oH

o

oH

oH

oH

o

oH

o

o o

oH

o

o

o

OH

Glucose

oH

oH

OH

oH

OH

Gallotannins Ellagitannins

Catechin

slide5
Cardiovascular disease, principally heart disease and stroke, are the leading cause of mortality in both men and women in all racial and ethnic groups.
  • The risk of atherosclerosis is increased by high blood pressure, hypertension, kidney disorders, obesity, diabetes, smoking, excessive alcohol consumption, stress, thyroid and adrenal gland problems, and lipid disorders

(Crespy and Williamson 2004)

slide6
Tea contains many compounds, especially poly-phenols and epidemiologically studies show that polyphenolic compounds present in tea reduces the risk of a variety of diseases.
  • Catechins are the main compounds in green tea; they consist of (-)-epicatechin, (-)-epicatechin-3-gallate (ECg), (-)-epigallocatechin, (-)-epigallocatechin-3-gallate (EGCg) etc.

(Graham, 1992)

slide7

Monomers (catechin, epicatechin, gallic and ellagic acid) of Tannin present in red wine/ green tea improve the antioxidative / immune status of the animals/ human.

  • (Percival et al., 2002)
  • Epidemiological studies shows that poly-phenolic compounds present in the Tea reduce the risk of a variety of diseases.
therapeutic values of tannin metabolites
THERAPEUTIC VALUES OF TANNIN METABOLITES
  • Mice consumed wine rich in phenolic components as catechin, epicatechin, gallic acid, ellagic acid showed increased antioxidant activity.
  • Gallic acid (GA) also showed strong antioxidant activity by preventing lipid per-oxidation.
  • GA is a strong antioxidant that possesses antimutagenic and anticarcinogenic activities

(Shahrzad et. al., 2001)

slide9
Green tea contains:

(+) – gallocatechin (GC),

(-)– epicatechin (EC),

(-)– epigallocatechin (EGC),

(-)– epicatechingallate (ECG),

(-)– epigallocatechingallate (EGCG)

which shows numerous biological activities including antibacterial, antioxidant, anti-tumor and cancer preventive activities.

  • Catechin serve as powerful antioxidant against lipid per oxidation when phospholipid bi-layers are exposed to aqueous oxygen radicals.
slide10
(+)Catechin prevents cancer (liver, lung, breast and colon cancer) in at least one of three ways:

First, they can prevent the formation of carcinogens,

Second, they tone up the body's natural detoxification defenses, and

Finally, they suppress cancer promotion.

  • (+)Catechin has other promising qualities:

antibacterial and antiviral agent, regulating cholesterol and blood pressure, and reducing blood clotting tendencies that may cause heart attacks or strokes

slide11

NEUTRACEUTICAL EFFECTS OF CATECHIN AND EPICATECHIN

  • Powerful Antioxidant effect – prevent lipid peroxidation
  • (Terao et al., 1994).
  • Antioxidant activity
  • Scavenge free radicals and reduce oxidative stress.
  • Enzyme induction and enhancing detoxification
  • glutathione peroxidase
  • catalase
  • superoxide dismutase
  • Catechin and epicatechin have Super Oxide Dismutase
  • (SOD) like activity.(Kim et al., 1995)
slide12

Cont…

Prevent free radical induced RBC hemolysis.

(Zhang et al., 1997)

Prevent coronary artery disease.

(Ishikawa et al., 1997)

Inhibit the oxidation of LDL cholesterol, reduced

body fat and thereby decrease incidence of heart

disease.

Catechins may stimulate the secretion of Bile

salts and the fecal excretion of cholesterol.

slide13
EGCG inhibit phospholipid hydro peroxidation in plasma.
  • Catechin have an anti-hyperglycemic action (Liu et al., 2005)
  • Lowering blood glucose and normalizing insulin release.
  • Prevent cancer(Valcic et al, 1996) – prevent formation of carcinogen, increase the body’s detoxification mechanism against tumor inducing agents.
slide14
Inhibition of Onco-gene expression

(Liu, 2004)

  • Gallic acid occurs as a free molecule or as part of a tannin molecule.
  • GA is a strong antioxidant that possesses antimutagenic and anticarcinogenic activities

(Shahrzad et. al., 2001)

slide15
Weight gain, food intake and liver weight of the rates on control and powered green tea (PGT) treatments.(Zhang, et al., 2002)
slide16

Effect of dietary powered Green tea on serum cholesterol level and Atherogenic Index (A) and on serum triglyceride and lipid peroxide level (B).(Zhang, et al., 2002)

A

1. Total cholesterol m mol / l

2. HDL - Ch

3. VLDL + LDL - Ch

4. Atherogenic index

B

1. Serum triglyceride

2. Serum lipid peroxide

slide17

Plasma cholesterol, TG and oxidative state of plasma lipoprotein in mice fed on atherogenic diet with and without tea extract. (Miura, et al., 2001)

slide18

Atheromatous area, aortic weights and aortic lipid contents in mice fed an atherogenic diet with and without tea extract.(Miura, et al., 2001)

slide20
Varying level of Potency among Catechins.
  • A in vivo experiment indicated that the components of tea catechins possess varying inhibitory effects on Cu2+ - mediated oxidation of LDL in the following order:

EGCg > ECg > EC > C > EGC

(Miura, et al., 1994 & 2001)

slide21

Catalase protein expression in rats at 16 wks of age that consumed water (control), Black tea polyphenols, Green tea polyphenols for 3 wks (Negishi, et al., 2004)

Extract was having tea Polyphenols 5 g /l of water.

And water consumption was 30 ml /d

slide24

Composition of Phenolic Compounds in Muscadine Wine (Percival et al.,2002)

Mg/Liter Muscadine Wine

Total Phenols 834

Gallic acid 14

Ellagic acid 8

Catechin 5

Epicatechin 22

B3 procyanidin 0.3

total antioxidant capacity of plasma of mice consuming red muscadine wine or ethanol
Total antioxidant capacity of plasma of mice consuming red muscadine wine or ethanol

1 : Muscadine wine

2 : Ethanol (Percival et al.,2002)

slide26
Catechins hypothesized to help protect against various diseases by contributing, along with antioxidant vitamins (i.e. Vit. C & E) and enzymes (i.e. SOD and Catalase)
  • In vivo studies show that green tea catechins increase total plasma antioxidant activity.
  • Intake of Green tea extract also increases the activity of SOD in serum and the expression of catalase in the aorta.
  • Malondialdehyde, marker of oxidative stress, decreases after Green tea intake
slide27
Catechins have a direct (antioxidant) or indirect (increase of activity or expression) effect.
  • Because catechins can act as antioxidant in vitro, they might prevent the oxidation of other antioxidants, such as vitamin E.
  • In some cases ingestion of green tea catechins does not modify the plasma status of vitamin E and C in vivo
  • Catechins increase vitamin E concentration in LDL and in this way could protect LDL against peroxidation.
slide29
In an oral Glucose tolerance test in rats, tea catechins decreased plasma insulin levels but did not affect plasma glucose levels.
  • In type 2 diabetes, lipid metabolism is modified: Plasma and Liver triglyceride levels and plasma cholesterol levels are elevated, GTE intake reduced these values in rats fed a sucrose-rich diet.
  • Catechins also reduced plasma TG levels.
slide30
Tannic acid (TA) induces glucose transport through activation of the Insulin-mediated signaling pathways in adipocytes.
  • TA inhibited the expression of key genes for adipogenesis, and has an insulin-like glucose transport stimulatory activity in adipocytes.
  • Epigallocatechin gallate (EGCG) enhances oral glucose tolerance in severely diabetic mice and in moderately diabetic rats.
conclusion
CONCLUSION
  • Tannins & its monomers have profound effects on health.
  • Not advisable to take a large quantity of tannins, as they may be toxic.
  • It is important to determine the correct dosage of tannins for promoting optimal health.