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Model-Driven Engineering of RNA Devices to Quantitatively Program Gene Expression

Model-Driven Engineering of RNA Devices to Quantitatively Program Gene Expression. James M Carothers, Jonathan A Goler , Darmawi Juminaga , Jay D Keasling Presented by Lauren Berry April 25, 2012. Goal. RNA-regulated devices with predictable activity Allow programmable pathways/circuits

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Model-Driven Engineering of RNA Devices to Quantitatively Program Gene Expression

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  1. Model-Driven Engineering of RNA Devices to Quantitatively Program Gene Expression James M Carothers, Jonathan A Goler, DarmawiJuminaga, Jay D Keasling Presented by Lauren Berry April 25, 2012

  2. Goal • RNA-regulated devices with predictable activity • Allow programmable pathways/circuits • Ribozyme-regulated expression devices (rREDs) • Aptamer-regulated expression devices (aREDs)

  3. Design-Driven Engineering

  4. Identify Functional Designs Impact of Design Variables

  5. Identify Functional Designs Impact of Design Variables

  6. Physical Implementation Design Method

  7. Physical Implementation Folding Characterization

  8. Assembly & Verification Expression Profiles

  9. Assembly & Verification Predicted vs. Observed Expression

  10. Conclusions • Expression mediated by rREDs and aREDs correlated with the predicted levels • Folding was important to programmable function • Functional aREDs and rREDs can be made from component parts

  11. Significance • Program expression of large number of genes Can be used to: • Study natural mRNA activity • Control engineered pathways or circuits • Implement new genetic programs

  12. Concerns • Not much description on rRED/aRED mechanism • Only characterized either rREDs or aREDs, not both • Essential information only in supplemental material

  13. Questions?

  14. Identify Functional Designs The System

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