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Efficient Gene Transformation Protocol for ScaI-Cut Strains

Discover a reliable procedure for gene transformation in ScaI-cut strains using ScaI probe SANK-18767. This protocol involves laeA1, laeA2, Hygr, Ppgk, Tpgk, and pSAK-ExplaeA1/laeA2 constructs. Obtain successful transformants with detailed steps and tips provided.

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Efficient Gene Transformation Protocol for ScaI-Cut Strains

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  1. A 2.8 kb ScaI ScaI Probe SANK 18767 laeA1 ScaI Hygr Ppgk Tpgk laeA1/laeA2 ScaI pSAK-ExplaeA1/laeA2 ScaI ScaI ScaI ScaI 2.2 kb 5.5 kb Hygr Transformant B C + laeA2 + laeA1 +laeA1 +laeA2 M 4 3 2 1 P 4 3 2 1 (kb) 5.5 2.2 D +laeA1 +laeA2 Fig. S3

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