Laboratory Safety

1. Laboratory Safety Christos Tsatsanis http://molmedgp.med.uoc.gr

3. Safety clothing: Labcoats

4. Protect ourselves

5. Protect our experiments

6. Safety Signs Mandatory Signs Hazard warning Signs Danger Signs Prohibition signs Compressed gas signs

9. SMOKING-Strictly Prohibited!Fire Hazard, Health Hazard, may affect your experiments

15. Dangerous for the environment Corrosive Flammable Harmful Irritant Explosion Hazard

16. Oxidizing agent (O) Toxic Very Toxic National Fire Protection Association's label

25. Toxicity- acrylamide toxicity Major HazardsSuspected human carcinogen (OSHA "select carcinogen") and neurotoxin. ToxicityThe acute toxicity of acrylamide is moderate by ingestion or skin contact. Skin exposure leads to redness and peeling of the skin of the palms. Aqueous acrylamide solutions cause eye irritation; exposure to a 50% solution of acrylamide caused slight corneal injury and slight conjunctival irritation, which healed in 8 days. The chronic toxicity of acrylamide is high. Repeated exposure to ~2 mg/kg per day may result in neurotoxic effects, including unsteadiness, muscle weakness, and numbness in the feet (leading to paralysis of the legs), numbness in the hands, slurred speech, vertigo, and fatigue. Exposure to slightly higher repeated doses in animal studies has induced multisite cancers and reproductive effects, including abortion, reduced fertility, and mutagenicity. Acrylamide is listed in IARC Group 2B ("possible human carcinogen") and is classified as a "select carcinogen" under the criteria of the OSHA Laboratory Standard.

27. Chemical hoods

28. http://www.hhmi.org/about/labsafe/index.html http://web.princeton.edu/sites/ehs/labsafetymanual/index.html http://www.ehs.umass.edu/lhs.html

37. v.1.0 January 99

42. Discard and Decontaminate culture fluids, cells etc. • Decontamination is achieved using Clorine at a concetration of 20%

49. 3. Laboratory Damage - Cornell(note shelving torn from the wall)