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Autoanalyzer

Autoanalyzer. Prof. Moustafa M. Mohamed. Autoanalyzer system. The autoanalyzer sequentially measures blood displays this on a graphical readout. This accomplished by mixing, reagent reaction, and colorimetric measurement in a continuous stream. Mixing or Proportioning Pump & Modifier.

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Autoanalyzer

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  1. Autoanalyzer Prof. Moustafa M. Mohamed

  2. Autoanalyzer system • The autoanalyzer sequentially measures blood displays this on a graphical readout. • This accomplished by mixing, reagent reaction, and colorimetric measurement in a continuous stream.

  3. Mixing or Proportioning Pump & Modifier Sample Colorimeter Heating bath Dialyzer Graphic Recorder

  4. Autoanalyzer system included the following elements:- • Sampler- aspirates sample, standards, and wash solutions to the autoanalyzer system. • Proportioning pump and manifold- introduces (mixes) samples with reagents to effect the proper chemical reaction to be read by the colorimeter. It also pumps fluids at precise flow rates to other modules, as the proper colour development depends on reaction time and temperature

  5. Dialyzer- separates interfacing substances from the sample material by permitting selective passage of sample components through a semi permeable membrane. • Heating bath- heats fluids continuously to exact temperature (typically 37oC incubation equivalent temperature). Temperature is critical to colour development

  6. Colorimeter- monitors the changes in optical density of the fluid stream flowing through tubular flow cell. Colour intensities (optical densities) proportional to substance concentrations, are converted to equivalent electrical voltages. • Recorder- converts optical density electrical signal from the colorimeter into a graphic display on a moving chart.

  7. The heart of the autoanalyzer system is the proportioning pump. • This consists of a peristaltic (occluding or roller) pump • Air segmentation in mixing tube separates the sample/reagent mixture from the cleaning fluid and other samples. • As these air-separated fluids transverse the coil of the mixing tube, effective mixing action is achieved.

  8. One problem with automatic analyzers is contain identification of samples. • Patient data can be intermixed with that of other patients if care is not taken. • Sterilization is also needed for samples, glassware. And equipment parts that are contaminated with disease. • Disease such as hepatitis or other communicable infections can be spread to equipment operators.

  9. Maintenance • Frequent calibration adjustment. • Most problems are mechanical (tubes, moving pump parts) and electrical (switches, motors). • Electronic failures are few. • Operation and service manuals must always be consulted.

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