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Bacterial Artistry: Capturing Life with Light Through Bacterial Photography

Explore the fascinating world of bacterial photography, where engineered E. coli create stunning visual imprints. Learn the steps involved in this innovative process, from mixing bacteria to incubation. Discover the intricate two-component signaling system that drives communication between sensor and responder molecules. Dive into the pulse-on, pulse-off mechanism and the potential challenges faced in the system, offering room for improvement and future innovations.

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Bacterial Artistry: Capturing Life with Light Through Bacterial Photography

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  1. BLI Research and Design Project Richa Panara

  2. Bacterial Photography http://www.pwponline.org/blog/2010/08/15/imprints-bacterial-photography-by-jeff-tabor/

  3. Steps Involved in the Production of Bacterial Photographs • Mix 1 million engineered E. Coli into a warm molten solution of a growth medium. • Cast the solution into a common petri dish and set it at room temperature to ensure it solidifies into a film. • Place the film inside an incubator and shine a focused image of light onto the surface of the film. • Grow the bacteria for approximately 12 hours. • After the 12 hours, remove the bacterial film from the incubator.

  4. 2 Component System Two component signaling pathway The sensor and the responder molecules The communication between the two component signaling pair occurs via a three-reaction “phosphorelay”

  5. Phosphorelay http://openwetware.org/wiki/20.109(F12):_Mod_2_Day_2_Measuring_system_performance • Sensor • Autophosphorylation • Histidine protein kinase • Phosphorylation of responder • Regulation of relevant genes • Reset the system

  6. The System http://openwetware.org/wiki/20.109(F12):_Mod_2_Day_2_Measuring_system_performance http://openwetware.org/wiki/20.109(F12):_Mod_2_Day_2_Measuring_system_performance

  7. Pulse-On, Pulse-Off Bacteria http://2012.igem.org/files/presentation/Tokyo-NoKoGen.pdf

  8. The System

  9. Toggle Switch

  10. The System

  11. Expectations, Problems, and Testing • Potential Problems • Longer activation time than expected • Kill switch may not function • Frequency may not be accurate • Room to Improve? • A gene that is more sensitive to sound • a protein or operon that produces a greater amount/power of luminescence

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