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BLI Research and Design Project

BLI Research and Design Project

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BLI Research and Design Project

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  1. BLI Research and Design Project Richa Panara

  2. Bacterial Photography http://www.pwponline.org/blog/2010/08/15/imprints-bacterial-photography-by-jeff-tabor/

  3. Steps Involved in the Production of Bacterial Photographs • Mix 1 million engineered E. Coli into a warm molten solution of a growth medium. • Cast the solution into a common petri dish and set it at room temperature to ensure it solidifies into a film. • Place the film inside an incubator and shine a focused image of light onto the surface of the film. • Grow the bacteria for approximately 12 hours. • After the 12 hours, remove the bacterial film from the incubator.

  4. 2 Component System Two component signaling pathway The sensor and the responder molecules The communication between the two component signaling pair occurs via a three-reaction “phosphorelay”

  5. Phosphorelay http://openwetware.org/wiki/20.109(F12):_Mod_2_Day_2_Measuring_system_performance • Sensor • Autophosphorylation • Histidine protein kinase • Phosphorylation of responder • Regulation of relevant genes • Reset the system

  6. The System http://openwetware.org/wiki/20.109(F12):_Mod_2_Day_2_Measuring_system_performance http://openwetware.org/wiki/20.109(F12):_Mod_2_Day_2_Measuring_system_performance

  7. Pulse-On, Pulse-Off Bacteria http://2012.igem.org/files/presentation/Tokyo-NoKoGen.pdf

  8. The System

  9. Toggle Switch

  10. The System

  11. Expectations, Problems, and Testing • Potential Problems • Longer activation time than expected • Kill switch may not function • Frequency may not be accurate • Room to Improve? • A gene that is more sensitive to sound • a protein or operon that produces a greater amount/power of luminescence