slide1 n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Besprozvannykh V.V ., Ermolenko A.V ., Atopkin D.M. PowerPoint Presentation
Download Presentation
Besprozvannykh V.V ., Ermolenko A.V ., Atopkin D.M.

Loading in 2 Seconds...

play fullscreen
1 / 18

Besprozvannykh V.V ., Ermolenko A.V ., Atopkin D.M. - PowerPoint PPT Presentation


  • 190 Views
  • Uploaded on

Trematodes of the family Haploporidae Nicoll, 1914 from mugilid fishes of Primorye region: ribosomal genes sequence data of the genus Skrjabinolecithum Belous, 1954. Besprozvannykh V.V ., Ermolenko A.V ., Atopkin D.M. Institute of Biology and Soil Sciences FEB RAS Vladivostok.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Besprozvannykh V.V ., Ermolenko A.V ., Atopkin D.M.' - addison-hickman


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide1

Trematodes of the family Haploporidae Nicoll, 1914 from mugilid fishes of Primorye region: ribosomal genes sequence data of the genus Skrjabinolecithum Belous, 1954

Besprozvannykh V.V., Ermolenko A.V., Atopkin D.M.

Institute of Biology and Soil Sciences FEB RAS

Vladivostok

systematic position of the genus skrjabinolecithum
Systematic position of the genus Skrjabinolecithum
  • TypePlatyhelminthes
  • ClassTrematoda
  • SubclassDigenea
  • FamilyHaploporidae
  • SubfamilyWaretrematinae
  • GenusSkrjabinolecithum Belous, 1954
  • Species:
    • S. spasskii Belous, 1954
    • S. indicum (Zhukov, 1972)
    • S. vitellosum (Martin, 1973)
    • S. lobolecithum (Martin, 1973)

Type species fromLiza haematocheila, Razdolnaya River, Primorye region

slide3

A

B

Skrjabinolecithum spasskii: А – mature specimen (original data), B – mature specimen from Belous (1954).

slide4

Hapalotrema flecterotestis Zhukov, 1971

Trematodes of Skrjabinolecithum: S. flecterotestis (= H. flecterotestis Zhukov, 1971; P. flecterotestis (Zhukov, 1971) Overstreet et Curran, 2005; Carassotrema flecterotestis (Zhukov, 1971) Yiu, Peng et Liu, 2005: А – original data, B – fromZhukov (1971), C – fromOverstreet & Curran (2005).

PlatydidymusflecterotestisOverstreet & Curran (2005).

Skrjabinolecithum flecterotestis

А

B

C

Skrjabinolecithum sp. nov. 1 – new species, described from Liza haematocheila

Skrjabinolecithumindicum– from Vietnam (Cat Ba Island) from Valamugilseheli.

slide5

The aim of this study isto confirm the validity of the species Skrjabinolecithum from Russian Far East and Vietnam using molecular data.

  • Sequencing of partial 28S rRNA gene and ITS1-5.8S-ITS2 rDNA fragment of investigated Skrjabinolecithum species;
  • Estimation of intra- and interspecific genetic differntiation of Skrjabinolecithum species using p-distances.
  • Reconstruction of the phylogenetic relationships of the genus Skrjabinolecithum.
slide7

18S

ITS1

ITS2

28S

IGS

5.8S

Amplification and sequencing of partial 28S rRNA gene and ITS1-5.8S-ITS2 rDNA fragment

D1-D3

5.8S

D3

ITS2

ITS1

D1

D2

slide8

RESULTS

Characterization of 28S rDNA sequences of different speciesof the genus Skrjabinolecithum

V – variable sites;

Pi – parsimony-informative sites;

S – singleton sites;

RFE – Russian Far East

slide9

Genetic differentiation of Skrjabinolecithum species by 28S rRNA gene sequences (amount of variable sites – above diagonal, p-distances, % - below diagonal). RFE – Russian Far East.

slide10

Maximum likelihood phylogenetic tree of the genus Skrjabinolecithum based on 28S rRNA gene sequence data.

Nodal numbers – statistical support for ML/BI algorithms.

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

I

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

96/0.97

Skrjabinolecithum spasskii RFE

Skrjabinolecithum spasskii RFE

93/0.87

Skrjabinolecithum spasskiiRFE

Skrjabinolecithum spasskiiRFE

Skrjabinolecithum spasskiiRFE

II

87/0.60

Skrjabinolecithum spasskiiRFE

Skrjabinolecithum spasskii Vietnam

99/1.00

Skrjabinolecithum spasskii Vietnam

66/0.93

Skrjabinolecithum spasskii Vietnam

Skrjabinolecithum spasskii Vietnam

Skrjabinolecithum sp. nov. 1RFE

69/0.31

III

Platydidymus flecterotestis RFE

70/1.00

Platydidymus flecterotestis RFE

61/0.53

Platydidymus flecterotestis RFE

Platydidymus flecterotestis RFE

IV

Skrjabinolecithum indicum Vietnam

99/1.00

Skrjabinolecithum indicum Vietnam

81/0.96

Skrjabinolecithum indicum Vietnam

AY222266 Pseudomegasolena ishigakiense

99/1.00

AY222267 Atractotrema signai

slide11

100

S. spassskii RFE

100

S. spasskii RFE+Vietnam

100

100

S.sp.nov.1

100

P. flecterotestis RFE

61

64

72

S. indicum Vietnam

97

100

EF032696 Saccocoelioides sp.

FJ211239 Forticulcita gibsoni

FJ211237 Haploporus benedeni

100

100

FJ211236 Lecithobotrys putrescens

FJ211262 Dicrogaster contracta

100

100

FJ211261 Dicrogaster contracta

100

FJ211238 Dicrogaster perpusilla

FJ211260 Saccocoelium obesum

99

100

100

FJ211259 Saccocoelium obesum

63

FJ211234 Saccocoelium brayi

FJ211233 Saccocoelium cephali

100

73

FJ211258 Saccocoelium tensum

99

FJ211257 Saccocoelium tensum

100

AY222265 Hapladena nasonis

AY222266 Pseudomegasolena ishigakiense

AY222267 Atractotrema signai

DQ836244 Paragonimus westermani

Waretrematinae

Chalcinotrematinae

Forticulcitinae

Haploporinae

Megasoleninae

Maximum likelihood phylogenetic tree of the family Haploporidae based on 28S rRNA gene sequence data.

slide12

d = 0.9 – 4.8% (genus Saccocoelium, from Blasco-Costa et al., 2009)

d = 9.6 – 15.8% (familyHaploporidae, from Blasco-Costa et al., 2009)

12.7%

?

P. flecterotestis

12.7%

0.12%

S. indicum

S. sp. nov. 1

0.8%

0.7%

12.7%

S. spasskii

slide13

Maximum likelihood phylogenetic tree of S. spasskii based on 28S sequence data. Statistical support showed for ML/BI algorithms. RFE – Russian Far East

Skr3_12

Skr3_13

Skr3_15

Skr3_16

Skr3_18

Skr4_1

100/0.63

Skr4_10

Skr4_11

Genotype 1

RFE

Skr4_12

Skr4_13

P1

100/1.0

P2

P3

P4

0.4%

Skr3_17

0.5%

Skr4_2

Skr4_9

100/0.62

Skr4_15

100/1.0

Genotype 2 (RFE)

S.spas34_1

S.spas34_2

S.spas34_3

S.spas34_4

100/0.95

0.1%

SkrV1

SkrV2

SkrV3

Genotype 3

(Vietnam)

KC206500_Spiritestis herveyensis

100/1.0

KC430096_Intromugil mugilicolus

KC430095_Intromugil alachuaensis

0.02

d = 0.9 – 4.8% (genusSaccocoelium, from Blasco-Costa et al., 2009)

d = 2.7 – 2.8% (genus Intromugil, from Pulish & Overstreet, 2013)

slide14

Maximum likelihood phylogenetic tree of S. spasskii based on ITS1-5.8-ITS2 sequence data. Statistical support showed for ML/BI algorithms. RFE – Russian Far East.

Skr3_12

Skr4_10

SkrP1

Skr4_12

SkrP2

82/1.0

Skr3_18

SkrP4

SkrP3

Skr4_13

Genotype 1 (RFE)

99/1.0

Skr4_11

Skr3_17

Skr3_11

Skr4_9

100/1.0

0.7%

Skr4_15

Skr34_4

0.9%

Skr34_2

SkrV3

Genotype 2 (RFE)

SkrV1

SkrV2

Skr34_1

0.3%

Skr34_3

KC430096_Intromugil mugilicolus

100/1.0

KC206500_Spiritestis herveyensis

Genotype 3 (Vietnam)

0.1

d = 2.1 – 10.9% (genusSaccocoelium, from Blasco-Costa et al., 2009)

slide15

Genetic differentiation ofS. spasskii

Variable nucleotide sites with fixed substitutions of partial 28S rRNA gene (1047 bp) sequences ofS. spasskii from Russian Far East and Vietnam. Gray colored cells – variable nucleotide sites for Genotype 2 (RFE).

Variable nucleotide sites with fixed substitutions of ITS1-5.8S-ITS2 (1511 bp) sequences ofS. spasskii from Russian Far East and Vietnam. Gray colored cells – variable nucleotide sites for Genotype 2 (RFE).

slide16

Hypothesis of genetic differentiation events of S. spasskii

Primorye Region

Razdolnaya River

China

Primorye Region

Mixed variants of S. spasskii rDNA sequences

Kievka River

S. spasskii d=0.5/0.9%

Vietnam, Cat Ba

Liza haematocheila

slide17

Platydidymusflecterotestisis closely related to S. spasskii by 28S rDNA with genetic differentiation 0.8% that represents minimal interspecific divergence level (Blasco-Costa et al., 2009). These data confirmed the validity of Platydidymusflecterotestisand support its membership to the genus Skrjabinolecithum.

  • Molecular variation of ribosomal DNA sequences with fixed point substitutions was revealed within species S. spasskii (from Liza haematocheila) obtained from different geographical locations. Three genotypes of S. spasskiirevealed by these data. Genetic differentiation of Far Eastern and Vietnamese S. spasskiiwas most likely due to geographic isolation.
  • Mixed variants of S. spasskiiribosomal DNA sequences from the same definitive host specimen can be explained by two main events: 1 - geographic isolation of the first intermediate host species (mollusks) in the past and 2 - migration or introduction of infected Liza haematocheilafrom Vietnam to the Russian Far East;
  • The question about taxonomical status of S. sp.n.1 and S. indicum is need to be investigated.