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Shape of the Dose-Response Relationship. Why LC50?. . . . Concentration (or Dose). Response. Low. High. 0%. 100%. Acute Toxicity Test Design. Test Material (toxicant)PureCommercial formulationMixtures of known concentrationCarriers/solventsUnknown mixtures (eg. sediment,effluent). 2. Test OrganismMost sensitiveMost representativeWild speciesRear in labKnown physiologyBred for uniformityCertified disease freeKnown susceptible strain.

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