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Pumpkin Purification

Pumpkin Purification

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Pumpkin Purification

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  1. Pumpkin Purification Removal of Toxic Metals from an Aqueous Solution Using Curcurbita Agricultural Waste Products Lauren Hodge Dallastown Area High School

  2. INTRODUCTION IDEA FORMATION • Review of Journal Article • York Flood • Garden Observation

  3. INTRODUCTION • Why do banana peels work? • Compounds in pumpkin • Hypothesis

  4. INTRODUCTION • Purpose of this research • Dosage dependent? • Lead versus chromium? • Is concentration a factor?

  5. RESEARCH Graphs courtesy of the United States Geological Survey

  6. RESEARCH WATER POLLUTION: A Problem Limited to Developing Countries?

  7. RESEARCH WATER POLLUTION: A Problem Limited to Developing Countries? INCORRECT!!!!!! • Exists globally, nationally, and locally • 12,531 pounds of toxic metals flow through the Susquehanna daily

  8. RESEARCH CURRENT REMOVAL METHODS • Chemical Precipitation • Ion Exchange • Reverse Osmosis • Phytoremediation • Adsorption- activated carbon, resins

  9. RESEARCH AGRICULTURAL WASTE PRODUCTS • Apple Waste • Corncobs • Cereal Crops • Barks and Leaves • Sawdust • Banana Peel

  10. RESEARCH CHARACTERISTICS OF SUCCESSFUL BIOMASS • Lignocellulosic biomass: plant biomass that contains cellulose, hemicellulose, and lignon • Cellulose: organic structural component compound found in plants, polysaccharide with a linear chain • Hemicellulose: similar to cellulose but has shorter chains that branch • Lignon: organic polymer, gives the plants structure lignon cellulose hemicellulose

  11. RESEARCH CHARACTERISTICS OF SUCCESSFUL BIOMASS • Carboxylic groups: organic acid, has at least 1 carboxyl group • Phenolic groups: also organic, has phenolic ring Phenolic ring Carboxylic acid

  12. PROCEDURE Preparation of Pumpkin (Stage 1): • Four pumpkins were washed, stems and seeds removed, and sliced to uniform thickness • Pumpkin slices were dehydrated using residential oven for approximately four hours • After strips were thoroughly cooled, they were placed into blender and pulverized • Pumpkin powder was stored in sanitized, labeled glass jars • 3.00 g of sieved pumpkin powder from each pumpkin was combined and mixed to create a homogeneous mixture of 12.0 g.

  13. PROCEDURE Preparation of Metal Ion Solutions (Stage 2): • 1 ppm solutions were prepared by pouring 4 ml of 1,000 ppm stock solution into 250 ml volumetric flask (done for lead and chromium) • 5 ppm were then prepared by pouring 20 ml of 1,000 ppm stock solution into 250 ml volumeteric flask (done for lead and chromium) • Deionized water was added to prepare solutions with total volume of 250 ml • This resulted in four solutions: 250 ml of lead in 1 ppm, 250 ml of lead in 5 ppm, 250 ml of chromium in 1 ppm, 250 ml of chromium in 5 ppm • Automatic pH probe was calibrated and solutions were titrated with sodium hydroxide in order to increase pH

  14. PROCEDURE Combining Pumpkin Powder and Metal Solutions (Stage 3): • 15 ml of each of the four metal solutions were poured into six labeled centrifuge tubes resulting in 24 experimental samples • Appropriate pumpkin dosage was then added (either 0.050 g or 0.50 g) • 13 additional control samples were also prepared • All samples were placed onto Orbital Shake Table overnight • Samples were centrifuged at 3000 rpm for five minutes • Samples were then vacuum filtered using 45 micron filter paper for 30 secs • Filtered samples were transferred to labeled ICP vials and run through ICP

  15. DATA

  16. DATA

  17. DATA

  18. DATA

  19. CONCLUSION • General Conclusion • Pumpkin Dosage

  20. CONCLUSION • General Conclusion • Pumpkin Dosage