Chemistry and Physics of Electrophoresis. Bio-Rad Biotechnology Explorer™ Dye/STEM Kit. Instructors - Bio-Rad Curriculum and Training Specialists. Sherri Andrews, Ph.D. email@example.com Damon Tighe, firstname.lastname@example.org Leigh Brown, M.A. email@example.com.
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Bio-Rad Biotechnology Explorer™ Dye/STEM Kit
Sherri Andrews, Ph.D.
Leigh Brown, M.A.
Electrophoresis means “to carry with electricity”
Buffer with charged ions
“For reasons not fully evident today, Tris became established as the favored cation for DNA Electrophoresis.” J.R. Brody, S.E. Kern/Analytical Biochemistry 333 (2004) 1-13
Dye Electrophoresis Commercial versus built box comparisons
V = Battery towerPhysics of Electrophoresis
V = IR
Side view of gel boxPhysics of Electrophoresis
V = Battery tower
R1 R2 R3
RTOT = 1
(1/(R1+R2+R3) + 1/R4)