Csi plant style from the laboratory to the wheat field
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“CSI” Plant Style: From the Laboratory to the Wheat Field. CAP Grant . Discover new, valuable, genes and QTLs Deploy them into adapted varieties around the country Use marker-assisted selection strategies to enhance gene deployment. CAP Grant.

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CAP Grant Field

  • Discover new, valuable, genes and QTLs

  • Deploy them into adapted varieties around the country

  • Use marker-assisted selection strategies to enhance gene deployment


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CAP Grant Field

  • 16 institutions around the country coordinate breeding efforts

  • 4 year grant started in 2006

  • Over 20 cultivars/germplasm already released

    (http://maswheat.ucdavis.edu)


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Impact of Wheat in FieldWashington In 2006

  • Excess of 2.2 million acres planted

    • 81% winter wheat, 19% spring wheat

  • Over 140 million bushels harvested

  • Net return of $615 million




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New Characteristics FieldNeeded

  • Drought tolerance

  • Disease and Insect resistance/tolerance

  • Herbicide resistance/tolerance

  • High yield

  • Excellent end-use quality (High GPC)


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DNA—Deoxyribo nucleic acid that carries the genetic information in a cell

Gene—a sequence of DNA that occupies a specific location and determines a particular characteristic

Definitions


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Definitions information in a cell

  • Allele-one member of a pair of genes that occupies a specific location on DNA

  • A= a=

    • = homozygous dominant

    • = homozygous recessive

    • = heterozygous


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Punett Square information in a cell


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Crossing information in a cell

  • Plants are identified which have the trait and which need the trait

  • Cross is made to introgress trait

    Survivor, no game X Rock-star yielder, dies

    10 Seeds (all )

    25 ( ) 50 ( ) 25 ( )

Self pollination


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Crossing information in a cell

  • Cross is made to introgress trait

    Survivor, no game X Rock-star yielder, dies

    10 Seeds (all )

    25 ( ) 50 ( ) 25 ( )

Self pollination

Survivor, Rock-star yielder?




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I am! Interested In?

I am!

I am!

I am!

I am!

I am!


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Definitions Interested In?

  • Molecular Markers—DNA segments that can be used as flags to track genes

  • Linkage—occurs when particular genes are inherited jointly

  • Linked Markers—markers which are closely associated with a gene


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DNA Interested In?

Gene

Gene

Marker

We know the DNA sequence of the marker

Sometimes we do not know the sequence of the gene we are looking for

We can use the marker to indirectly select for the gene of interest


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Tightly Linked Marker Interested In?

Gene

Marker

Gene

95% confident

Non-linked Marker

Marker

Gene

Gene

50% confident


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Marker-Assisted Selection Interested In?

  • Identify marker which is linked to gene

  • We can extract DNA from the plants and look for the presence of the marker

  • This will tell us which have the trait and which do not



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1 Interested In?

2

3

4


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+ 95% Ethanol Interested In?


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PCR Explanation Interested In?

  • Polymerase Chain Reaction

  • Used to amplify DNA via enzymatic replication

  • Allows a small amount of DNA to be used for analysis

  • PCR reaction

    http://www.danquinnart.com/wheatcap/index.htm


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Gel Electrophoresis Interested In?

  • Allows separation of PCR product

  • DNA inserted into agarose gel

  • DNA fragments travel with current

  • Smaller fragments will travel faster than large fragments


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Gel Analysis Interested In?


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Gel Analysis Interested In?


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Gel Analysis Interested In?


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Gel Analysis Interested In?


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Gel Analysis Interested In?



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Evaluation Interested In?

  • After MAS has identified which plants carry the trait, further evaluation is done in the field to make sure the plants are productive

  • Time to release a variety is shortened

  • Why would this be important?


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Zak Interested In?

+ 1 gene


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Tara 2002 Interested In?

Trait Selection

Crossing

From Laboratory to Wheat Field

Washington Farmers

Shepherds Grain

WSU Food Services


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Acknowledgements Interested In?


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