Tristan Ursell APh 162 California Institute of Technology. Beer’s Law and Spectrophotometry . Two-fold: - Relates concentration to the optical measurement of ‘absorbance’ - combined with spectrophotometry can be used to distinguish and compare different molecules in
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California Institute of Technology
Beer’s Law and Spectrophotometry
- Relates concentration to the optical measurement
- combined with spectrophotometry can be used to
distinguish and compare different molecules in
Uses of Beer’s Law:
Beer’s Law is stated in a way to make certain quantities easy to compare and interpret.
l – sample pathlength (usually 1cm)
c – concentration (mol/vol)
– molar absorption coefficient ( )
I – light intensity (W/m^2)
Rule of Thumb: A<1 for accurate results
Only at low concentrations, where the absorbance is linear (single scattering event):