7 Tips to Help Prevent Contamination in a Tissue Culture Lab

1. 7 Tips to Help Prevent Contamination in a Tissue Culture Lab Contamination control in plant tissue culture is one among the foremost difficult issues to manage, and therefore the real secret to your defense against contamination is prevention. Bacteria, mold, and mycoplasma are common contaminants that the majority lab personnel are conversant in throughout their work with tissue culture media. Bacterias are easily identified and may be addressed swiftly before they need an excessive amount of of a devastating effect.

2. Contaminants like mycoplasma and fungal pathogens usually leave fewer visual clues and may , therefore, have a more severe impact on your tissue culture if left unchecked. regardless of the contaminant, it's safe to mention they will be a nightmare to affect . In the case of tissue culture contamination, prevention is best than scrambling to eliminate. Use these seven tips to stay your experiments clean, healthy and freed from contamination. 1. Use PPE Wear gloves, lab-coats, and use hoods in order that there's a barrier between the non-sterile factors (yes that has you) and therefore the cells. Use personal protective equipment like lab coats and gloves. Have your hood cleaned and serviced regularly. This maintenance will help to make sure that your hood is functioning efficiently which your culture media is getting all the protection necessary. There are different sorts of hoods available, and every type offers a special level of protection. 2. USE THE HOOD PROPERLY If you followed the recommendation in the first step, then you've got a hood to guard your culture samples. But just having a hood isn't enough - you would like to use your hood correctly. Yes, this might seem obvious to you, but merely having a hood isn't enough to make sure a secure environment. The primary thing to see is that there's proper airflow inside the hood. Confirm that nothing is interfering with this airflow which nothing is covering air inlets or outlets. Cleanse the hood after each working session by wiping it down with sterilizing materials or using your ethanol solution. Although some people wish to have a waste disposal basket within the hood area, it could compromise the environment, so it's best to stay your waste disposal outside the hood. And at the top of the day, make certain to modify on the UV light inside the hood to enable overnight sterilization. 3. CLEAN YOUR INCUBATOR Some older model incubators don't have self-cleaning mechanisms. If this is often one among yours, then confirm that you simply manually clean the incubator consistent with its specific protocol. 4. SANITIZE, SANITIZE, SANITIZE Use 70% ethanol mixed with water to kill bacteria. Spray everything, including your gloves and all equipment before you bring it into the hood. If you're right dominant, keep your equipment to your right, your hood directly ahead , and therefore the ethanol disinfectant on your left in order that you'll devour the equipment together with your right , spray it together with your left , then place it inside the hood. Simple, streamlined, and efficient systems like these will prevent careless contamination. Wash your hands before you interact with the tissue culture media, and confirm that anything you're wearing is clean and sanitized. 5. USE PLANT PRESERVATIVE MIXTURE

3. PLANT PRESERVATIVE MIXTURE (PPM) not only targets and kills contaminants (including fungi), it also can prevent plant contamination from ever occurring. Use PPM™ with a high-quality medium to make sure healthy and guarded experiments. Plant preservative mixture may be a liquid concentrate that's effective against human contact, endogenous, waterborne, and airborne microbial contamination. 6. KEEP CELL EXPOSURE TO A MINIMUM Maintaining a sterile environment inside the hood and incubator is meaningless if you allow the cells exposed in an unsterilized environment. Yes, sometimes it's necessary when transferring between the incubators and hood or viewing under a microscope but plan in order that they spend the smallest amount of your time outside as possible. 7. KEEP ORGANIZED Even if your personal life is in shambles, confirm that your tissue culture lab isn’t. Following a dogmatic organizational approach is going to be your greatest asset within the lab. Label everything, and follow procedures right down to the previous detail. The only thing between your tissue cells and contamination may be a set of strictly followed techniques and procedures to take care of aseptic environmental conditions. If this sounds complicated, just remember to wear the acceptable protective equipment, sterilize your equipment and use plant preservative mixture to stop and treat contaminants.