miRNA sequencing for cancer

1. NEXT GENERATION SEQUENCING SERVICE DNA Library Preparation Clonal Amplification 1 2 Next-generation sequencing (NGS) technologies progressive advantages in terms of cost-effectiveness, unprecedented speed, high resolution and accuracy in genomic analy- ses, thus are playing an increasing important role in fields of oncology and immu- nology. have 1 2 3 sequencing ????????? 4 5 The sequencing principles of common sequencing instru- ments on the market: (1) first, construct a DNA template library. Obtain DNA library fragments (tens to hundreds of bases in randomly breaking genomic DNA, or construct paired-end fragments that control the distance distribution. Adapter sequences were ligated to both ends of fragment and then denatured to obtain a single-stranded template library and immobi- lized on a solid surface. (2) Second, amplify the clones, which performed by one of several methods, such as bridge PCR and emulsion PCR. (3) Then, forming DNA clusters or amplifying micro- spheres of DNA cluster arrays on a chip, performing a series of cyclic reaction operations using a polymerase\ or ligase, and monitoring the optical events generated in each cycle biochemical reaction by a microscopic detection syst- em. Time series analysis of the resulting array images is performed to obtain sequenc- es of DNA fragments. These fragments are then assem- bled into longer according to certain computer algorithms. ?????????? ????????????? length) by the DNA ???????????????? ???????????? Cyclic Array Sequencing 3 ??? ?????? 5' 3' ??? ?????? 5' 3' ??????????????? 3' 5' 3' 5' ???? ?????????? 3' 5' ??? T ??????????? A A A A T A T T T A T ????????? A A A ?????????? C A A A ??????????? ????????? A G ????????? ???????? ??????????????? ???????? ???????? ???? ??????????????????????? ?????????????? ??????? ?????????????????? G A C z A n z n n 3' ??????????? ?????? z ???????? z n z A C G T 3' n n A G C T G T A 3' C ?????????? 3' G A C A 3' C T contigs G ????????? ???? 3' ???????? ?????????? C T G T T ???????? z T G A A A A C A z z G A n n n 3' 3' 3' 5' 5' 5' Creative Biolabs uses the advanced SuPrecision™ pl- atform to support researchers all over the world with their sequencing needs for cancer. A A n n n z z z C A n n n z z z A T C T G T T T ??????? ??? 3' C T 3' 3' 5' G 5' T G C T C G A n n n z z z T A n n n z z z ?????????????????????? A C GG C A ????????????? WHATWEDO: Whole Genome Sequencing (WGS) Service for Cancer Whole Exome Sequencing (WES) Service for Cancer Targeted Sequencing Service for Cancer Creative Biolabs Next Generation Sequencing Service Whole Transcriptome Sequencing (WTS) Service for Cancer Immune Repertoire Sequencing (Rep-seq) Service for Cancer Bioinformatics Analysis Service © 2007 - 2019 Creative-Biolabs All Rights Reserved Email: info@creative-biolabs.com Tel: 1-631-381-2994