Introduction of Biotechnology. Chapter 7 Lecture 7-3 Microbial Genetic Engineering. Topics Useful Tools and Techniques Recombinant DNA Technology Recombinant microorganisms Genome Analysis Practical applications. Tools and Techniques. Enzymes as Tools DNA analysis
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Useful Tools and Techniques
Recombinant DNA Technology
Fig. 2.3.1 Some useful properties of DNA
Fig. 2.3.3 Revealing the patterns of DNA with electrophoresis
Fig. 2.3.4 A hybridization test using microbe-specific probes
A Southern blot separates DNA by electrophoresis, denatures and transfers the DNA to filter paper, and uses probes to visualize hybridization.
Fig. 2.3.5 Conducting a Southern blot hybridization test.
Fig. 2.3.6 Dideoxyadensine triphosphate (ddATP)
Fig. 2.3.7 Steps in a Sanger DNA sequence technique
Fig. 2.3.8 Diagram of the polymerase chain reaction
Practical applications of recombinant technology include the development of pharmaceuticals, genetically modified organisms, and forensic techniques.
Fig. 2.3.9 Methods and applications of genetic technology
Fig. 2.3.10 Partial map of the pBR322 plasmid of E. coli
Fig. 2.3.11 Plasmid map of the pJB8 cosmid of E. coli
Fig. 2.3.12 Yeast artificial chromosome with inserted human DNA
Enables large scale manufacturing of life-saving hormones, enzymes, vaccines
insulin for diabetes
human growth hormone for dwarfism
erythropoietin for anemia
Factor VIII for hemophilia
Fig. 2.3.13 Cloning and production of recombinant somatostatin in E. coli
Table 2.1 Current protein products from recombinant DNA technology
Fig. 2.3.14 Steps in recombinant DNA, gene cloning, and
Fig. 2.3.15 Bioengineering
Fig. 2.3.16 Mechanisms of antisense DNA and triplex DNA
Fig. 2.3.18 DNA fingerprints:the bar codes of life
Fig. 2.3.19 Pedigree analysis based on genetic screening
Fig. 2.3.20 Gene expression analysis using microarrays