Protein Analysis. Prof. Conlon ’ s Lecture Unit 9 - Molecular Medicine 22 nd Feb 2003 Week 2. 4MedStudents.com. Why do we analyse proteins?.
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Prof. Conlon’s Lecture
Unit 9 - Molecular Medicine
22nd Feb 2003 Week 2
How does it work?
Preperative (2.5-5cm) Analytical (0.4-1cm) Microbore (0.1-0.2cm)
Concavalin A is a glucose binding protein. It can be purified by passing it through a column that has special beads. These beads have glucose attached to them.
The glucose binding protein binds to the beads and other proteins don’t.
The protein can be released from the beads by increasing the concentration of glucose. This will displace the glucose binding protein from the beads.
Concavalin A Glycoprotein
Hormone Binding Protein/Receptor
Mass Spectrometry Diagram