HPG Axis. Target Cells. H ypothalamus. -. -. GnRH. LH & FSH. Testosterone. +. Anterior P ituitary. -. -. LH. FSH. Testosterone. Inhibin. +. +. Testosterone. Male G onads. Sertoli Cells. Leydig Cells. Hypogonadism. Hypogonadism
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LH & FSH
Foreign body reaction
Advantages of microencapsulation
Sustained release of hormones from entrapped cells
Reduced diffusion distance
Size exclusion via mesh size
LH, FSH, O2, Nutrients
OPolyethylene glycol (PEG)
10% PEGdA MW12000
± cell suspension
(365nm UV light)
Polymerization & cross-linking via free-radical mechanism
To investigate the effects of hydrogel thickness on the viability of human prostate cancer cells embedded within a polyethylene glycol diacrylate hydrogel. Additionally, to assess the polymerization and cross-linking phenomena of PEGdA macromers and the diffusive behavior of progesterone through a PEGdA hydrogel matrix.
The overall goal of this project is to design an encapsulation system that offers efficient immunoprotection and effective diffusion of oxygen, nutrients, hormones, and metabolic wastes.
This system, along with embedded human prostate cancer cells, will enable the restoration of un-regulated hormone levels commonly observed in elders, and retard the symptoms of aging.
14.5 minutes of UV exposure is sufficient for cross linking
3D Swelling Ratio = 3.8
Percent Change in Oxygen
Percent Change in Oxygen Concentration at Various Hydrogel
Thicknesses as Compared to the Oxygen Concentration
at the Site of Implantation
Distance (D) = (1/2) x [Time x Speed of Sound]
2-sample t-test α = 0.05
Cell Titer-BlueTM Cell Viability Assay
* Denotes significant drop from day 2 to day 3
* Denotes significant drop from day 3 to day 4
Albert Kwansa Eric Lee
John Harrison Miguel Benson
Client: Dr. Craig Atwood
Advisor: Professor William Murphy
Yik Ning Wong