Recombinant DNA Technology. 1971 paper by Kathleen Dana and Daniel Nathans described isolation of enzyme that cleaved DNA at specific sequences1978 Nobel Prize to Nathans, Smith and Arber for restriction endonuclease discovery. Cloning DNA Molecules. Purify DNA from desired sourceCut DNA with restriction endonucleaseJoin fragments to cloning vectors cleaved with compatible restriction endonuclease to create recombinant DNA moleculesTransfer recombinant molecules to host cellsIsolate DNA fro9455
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