2003 NELAC Standards Microbiology Checklist: How to “Pass” your Assessment. By Denise K. Williams Biological Scientist III Florida Department of Health Environmental Laboratory Certification Program FSEA Microbiology Workshop Rev. 2; 5-22-07. Do Your Homework.
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Denise K. Williams
Biological Scientist III
Florida Department of Health
Environmental Laboratory Certification Program
FSEA Microbiology Workshop
Rev. 2; 5-22-07
(Clean vents? Too close to wall?)
-Biological indicator: contains thermophilic spore-forming organism, typically Bacillusstearothermophilus
-Have you ever processed a non-autoclaved control? (See NELAC 184.108.40.206)
-Do you keep records? Optimally, these records would include the incubator ID, incubation period, traceability to lot of spores used, retention of spore certificates, analyst initials?
-Do you incubate at the proper temperature? Typically, 55-60oC (see manufacturer’s instructions).
-Includes sample bottles, sample bags, Quantitray.
-Did you record the lot number?
Note: These checks are not required for chlorinated water systems as long as :
Sufficient Na2S2O3 was added to each sample container to dechlorinate at least 5 mg/L Chlorine in Drinking Water samples & at least 15 mg/L Chlorine in Non-Potable Water samples.
-How do you demonstrate this?
Note: This blank must be analyzed prior to first use of the medium.
such as TSB, HPC, PCA, etc.]
Purchased Ready To Use:
**Pay attention to the required test intervals and the actual data!
NELAC 220.127.116.11.2 (b) Does each test method in the in-house methods manual include or reference: