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EP & TA Laboratories and Veterinary Diagnostics Ltd. 16 Phalirou Str. Ayios Pavlos 2362 Nicosia – Cyprus Tel. No. 00357 22 444 230 Fax No. 00357 22 444 231 Email: eptalab@gmail.com. EP & TA Laboratories ORGANOGRAM.
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EP & TA Laboratories and Veterinary Diagnostics Ltd 16 Phalirou Str. AyiosPavlos 2362 Nicosia – Cyprus Tel. No. 00357 22 444 230 Fax No. 00357 22 444 231 Email: eptalab@gmail.com
EP & TA Laboratories ORGANOGRAM • Dr Pavlos Economides D.V.M.-F.R.S.H Veterinarian-Bacteriologist – Director • Dr Savvas Gennaris D.V.M.-Veterinarian • Dr Marios Christoforou DVM-Msc – Veterinarian-Associate • Mr Tefkros Anaxagorou – Quality Manager-Chief Technician • Mrs Alkisti Gennari – Chemical Engineer • Mr Anaxagoras Anaxagorou – Electrical Engineer-Laboratory Technician • Mr Anaxagorou Andreas - Sampling
EP & TA Laboratories and Veterinary DiagnosticsActivities • VETERINARY DIAGNOSTICS • Microbiology • Parasitology • Serology • FOOD ANALYSIS • Bacteriology • Sensory Testing • Shelf Life Testing • Residues of inhibitory substances
TYPES OF SAMPLES EXAMINED • Animal Tissues – Blood samples • Foods of animal origin • Meat and meat products • Milk and milk products • Fish and fish products • Animal feeds • Water
FOOD ESTABLISMENT HYGIENE • Water supply – Bacteriological examinations-Chlorine residues • Evaluation of working surface Hygiene – Swab tests • Air samples - Bacteriology
METHODS FOR MICROBIOLOGICAL TESTING OF FOODS • Salmonellae ISO 6579-2002-Isolation – Identification - Serotyping • Escherichia Coli ISO 21528-2:2004 • Staphylococcus aureus ISO 6888-1:2003 • Listeria monocytogenes - Isolation – Identification ISO 11290-2:1998 • Colony-count- enumeration of microorganisms in animal feeding stuffs ISO 4833:2003 • Detection of Staphylococcal Enterotoxin in milk products – ELISA –Transia Kit
METHODS FOR MICROBIOLOGICAL EXAMINATIONS OF WATER • Detection and enumeration of Escherichia coli and Coliforms bacteria-Membrane filtration method ISO 9308-1 • Detection and enumeration of intestinal enterococci ISO 7899-2 • Total colony count ISO 6222 • Detection and enumeration of Faecal Coliforms ISO 9308-1 • Detection of Legionella spp ISO 11731
METHODS FOR MICROBIOLOGICAL TESTING OF SURFACES AND AIR SAMPLES SAMPLING Rodac-contact plates Hygicult Total Plate Count Agar & Hygicult E-Violet Red Bile Agar with addition of glucose TESTING • Total Plate Count ISO 4833:2003 • Detection and enumeration of Enterobacteriaceae ISO 21528-2:2004 • Detection of Salmonella spp ISO 6579-2002 • Detection and Enumeration of Listeria monocytogenes ISO 11290:1996
Bacteriological Examination • Cultures • Identification of Bacteria – Biochemical testing • P.C.R.-Polymerase Chain Reaction • Immunofluorescence
Polymerase Chain Reaction – Immunofluorescence Examination PCR • Legionella spp • Salmonellae spp • Listeria spp • Rickettsia • Coxiella • Leishmania • Bartonella Immunofluorescence • Legionella spp • Rickettsia • Coxiella • Leishmania • Bartonella
SEROLOGICAL TESTING • Standard Agglutination Tests • Complement Fixation Test • ELISA Tests
ELISA TESTS ELISA IN POULTRY • Infectious Bursal Disease – IBD • Infectious Bronchitis Virus – IBV • Newcastle Disease Virus – NDV • Avian Pneumovirus – TRT • Reovirus - REO • Mycoplasma gallisepticum - MG ELISA IN PIGS • Reproductive and Respiratory Syndrome – PRRS • Swine Influenza • Aujeszky Disease • Mycoplasma Hyopneumoniae • Porcine Parvovirus • Transmissible Gastroenteritis - TGE • Corona Virus
PARASITOLOGICAL TESTING • Microscopical examination • Parasites Egg Count by Floatation – McMaster • Protozoa
CUSTOMERS • Number of Farms 43 Types of Farms • Dairy Farms • Pig Farms • Poultry Farms • Rabbit Farms • Pets-dogs-cats • Cage birds • Number of Establishments 78 Types of Establishments • Poultry Slaughter houses • Slaughter houses for pigs, sheep and goats • Food establishments • Meat Cutting Plants • Meat Products • Dairies • Butcheries • Hotels • Restaurants
SALMONELLAE SEROTYPES ISOLATED List of most common Salmonellae serotypes isolated • Typhimurium • Braenderup • Brandenburg • Newport • Hadar • Derby • Anatum
LISTERIA SEROTYPES ISOLATED List of most common Listeria serotypes isolated • Listeria monocytogenes • Listeria innocua • Listeria welshimeri
SURVEILLANCE FOR ZOONOSES • Brucellosis • Rickettsioses • Coxiella • Bartonellosis • Leishmaniosis • Ehrlichiosis
COLLABORATION WITH OTHER INSTITUTIONS • World Health Organization – WHO network Salm-Surv as a General Institution Member for the GSS country Databank • University of Crete – School of Medicine - Laboratory of Clinical Bacteriology-Parasitology-Zoonoses and Geographic Medicine • WHO Collaborating Center for Research and Training in Mediterranean Zoonoses – Anogia - Crete • Royal Veterinary College London – Department of Pathology of Infectious Diseases - UK
PARTICIPATION IN INTERLABORATORY TESTS • We participate in all Interlaboratory testing organized by the National Reference Laboratory for foods of Animal Origin July 2004 – Total Colony Count in milk samples (accuracy d=-0,03) October 2004 - Total Colony Count in milk samples (accuracy d=-0,022) September 2005 – Detection of Salmonella and Listeria in cheese samples (accuracy 100%) May 2006 - Total Colony Count in milk samples (accuracy d=-0,009)
APPROVAL - LICENCED EP & TA Laboratories and Veterinary Diagnostics is approved and licensed by • The Department of Veterinary Services of Cyprus • The Veterinary Council The diagnosis tests of animal diseases, zoonotic agents and for the bacteriological examination of foods of animal origin including animal feeds. We are in the process for accreditation in accordance with the requirements of the ISO 17025:2005 standard.
