Heterodimers of G protein-coupled receptors. G protein-coupled receptors (GPCRs) exist as homodimers and also associate with other GPCRs to form heterodimers This association may alter the function of both receptors, yielding a distinct functional unit. examples.
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G protein-coupled receptors (GPCRs) exist as homodimers and also associate with other GPCRs to form heterodimers
g-aminobutyric acid B (GABAB) receptors that consist of GABAB1 and GABAB2 proteins that are not functional when either one is expressed alone
heterodimerization also leads to alterations in ligand specificity: dimerization of T1R1 with (T1R2 or T1R3)
Heterodimerization leads to a change in the binding pocket that could be specifically targeted to develop heterodimer selective drugs
In addition to dopamine and glutamate, the neuromodulator adenosine plays an important role in the function of striatal GABAergic efferent neurons
1. coimmunoprecipitation of both receptors
2.Bioluminescence energy transfer (BRET):the first protein partner is fused to Renilla luciferase and the second to a fluorescent protein.
BRET competition experiments were performed using a chimeric D2–D1 receptor in which the helices 5 and 6, the third intracellular loop (I3) and the third extracellular loop (E3) of the D2 receptor were replaced by those of the dopamine D1 receptor.
A2A receptor–D2 receptor heterodimers are preformed before being inserted into the plasma membrane they are probably subject to a dynamic regulation.
Finding out the mechanisms involved in this regulation can have important implications for the treatment of basal ganglia disorders, schizophrenia and drug addiction.