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Buffers play a crucial role in various biopharmaceutical processes such as protein isolation and purification, polishing, filtration, chromatographic operations, reactions, and crystallization steps. They are especially important in downstream processes, where they help protect proteins from fluctuations in pH. By absorbing or neutralizing changes in pH, buffers ensure that proteins remain stable. Despite variations in processing methods, container types, and raw materials, a well-formulated buffer solution will maintain its pH.
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