BCR Diagnostics. Bacteriological Biosensor for Rapid Screening of Platelets Shortly Before Transfusion. Bacterial Chain Reaction for Biomolecular Amplification. Boris Rotman, Ph. D. Vice President and CSO . Jamestown, Rhode Island - bcrbiotech.com. Technical Rationale.
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Bacteriological Biosensor for Rapid Screening of Platelets Shortly Before Transfusion
Bacterial Chain Reaction for Biomolecular Amplification
Boris Rotman, Ph. D.
Vice President and CSO
Jamestown, Rhode Island - bcrbiotech.com
Biosensor integrates three technologies:
1. LEXSASTM (Label-free Exponential Signal-Amplification System)
A recently developed system using engineered spores as fluorogenic nanodetectors.
The detection system is a unique device containing an array of 80,000 microscopic, independent biosensors, each of which is about 5-picoliter volume. This small reaction volume enables detection of individual bacterial cells. The device is termed “80K-bioChip.”
3. Computerized Parallel Imaging
The 80K-bioChip provides high-throughput data acquisition and processing.
Analyte + germinogenic buffer
Transduced Fluorescent Signals
DATA PROCESSING AND DISPLAY
Schematic of 80K-bioChip[Cross Section]
Platelet sample is processed to separate bacteria, and the
resulting suspension is mixed with engineered spores
Mixture is combined with germinogenic substrate and then filtered through the 80K-bioChip
80K-bioChip is incubated at 37oC
Fluorescent images of 80K-bioChip are captured at time intervals
Digital images are processed to yield number of bacteria in the sample
Micro-colanders with one bacterium show visible fluorescence
80K-BioChip showing ten (10) micro-colanders with significant fluorescence above baseline
Left: Normal light Right: Fluorescence light
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