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Third generation sequencing & Comparison of NGS Technology Platforms. Sravanthi Joginipelli. Available NGS technology platforms. Three commercially available NGS platforms, i.e. Second generation sequencing technologies 1. Roche (454) FLX Genome Sequencer, 2. Illumina Genome Analyzer II

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Presentation Transcript
available ngs technology platforms
Available NGS technology platforms

Three commercially available NGS platforms, i.e. Second generation sequencing technologies

1. Roche (454) FLX Genome Sequencer,

2. Illumina Genome Analyzer II

3. Applied Biosystems’ SOLiDTM (Sequencing by Oligo Ligation and Detection)

Third generation sequencing platforms

  • Helicos
  • HeliScope
  • Pacific Biosciences
third generation sequencing
Third generation sequencing

3rd generation

PacBio RS

  • Single Molecule Sequencing Technology (tSMS)
  • No amplification
  • 10Gb os sequence data per 8 day run
  • Single Molecule Real Time (SMRT) sequencing technology (PacBio RS)
roche illumina analyzers
Roche & illumina analyzers

Genome Sequencer FLX

Illumina Genome Analyzer

references
References
  • Dames S, Durtschi J, Geiersbach K, Stephens J, Voelkerding KV, Comparison of the Illumina Genome Analyzer and Roche 454 GS FLX for resequencing of hypertrophic cardiomyopathy-associated genes.
  • DNA Microarray Inc, “Genomic Services” http://www.dnamicroarray.com/services_genomics_ngs.html
  • Pacific Biosciences, http://www.pacificbiosciences.com/
  • 454 lifesciences http://www.roche-applied-science.com/