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Bioreactor Analysis and Operation Chapter 9&10 (textbook). Overview of bioreactors Modified batch and continuous reactors Scale up/down Immobilized cell systems Operation consideration Sterilization Bioreactor Instrumentation and control. Bioreactor Analysis and Operation.
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- solid state: water content: 40~ 80%, mostly mold fermentation on agriculture products and food: rice, wheat, barley, corn and soybean.
e.g.rotary drum fermentator
- submerged systems: water content > 95%
e.g. bacteria, yeast.
- batch: stirred tank
- continuous: chemostat, fluidized-bed
- modified types of the above modes: fed-batch, chemostat with recycle,
multi-stage continuous reactors
- aerobic: airlift
Glacial Lakes Energy in Watertown, South Dakota
47+ million gallon per year ethanol production .
World's Largest Industrial Fermenter (Chem. Eng. News,10-Apr-78)
The fermenter is 200' high and 25 ft diam.
Cell mass balance (qp=0, kd ≈0, X0=0,Monod equation is applied):
A chemostat can be operated at dilution rates higher than
the specific growth rate when cell recycle is used.
Mass balance on growth-limiting substrate (qp=0, kd ≈0, X0=0,Monod equation is applied):
µm=1.00h-1, S0=2.0g/l, Ks=0.01 g/l, Yx/s=0.5 g/g,
concentration factor C=2.0 and recycle ratio α=0.5
Organisms are cultured in a chemostat with cell recycle. The system is operated under glucose limitation.
Determine specific growth rate μnet, S in the reactor effluent, cell concentration in the recycle stream (CX1) and in the concentrator effluent (X2)
If the concentrator has a volume of 300 mL, what is the residence time in it?
Nutrients are continuously or semi-continuously fed, while effluent is removed discontinuously.
- overcome substrate inhibition or catabolite repression by intermittent feeding of substrate by maintaining low substrate concentration.
for production of secondary metabolites e.g. antibiotics, lactic acid, E. Coli making proteins from recombinant DNA technology.
Analysis of fed-batch with substrate continuously fed
and no output: t=0, V0=0, F is constant.
At quasi steady state, S added=S consumed,
X, S, P concentrations are constant.
Cell mass balance:
(Monod growth model applied)
where Xt=Xt0 at t=0
qp (i.e. g product/g cells-min)
at Pt=Pt0, t=0
In a fed-batch culture operating with intermittent addition of glucose, the value of V is given at time t=2hr, when the system is at quasi-steady state.
At t=2 hr, find S, X and Xt and P at quasi-steady state if qp=0.2 g product/g cells-h, P0=0.