Separation and Analysis of Honeybee Venom Components. Levi Blazer Liz Denning Laura Rhodes Juniata College Research Advisors: Dr. Lorraine Mulfinger and Dr. Michael Boyle. Honeybee Venom Components. Melittin: Our Primary Interest. Comprises 50% of raw honeybee venom
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
Dr. Lorraine Mulfinger and Dr. Michael Boyle
SEPHADEX® G-50 (MW 30,000 – 1,500)
Figure 1: Shows electrophoresis components
Figure: Shows PAGE gel results. (In lane 6: Whole Bee Venom, in lane 5: Melittin Standard, and in lane 1-4: Melittin Fractions)
1 2 3 4 5 6
1 2 3 4 5 6
Phospholipase A Fractions
Juniata College Dept. of Chemistry
Dr. Marielena McGuire
Field Scientist, Mid-Atlantic Region,
Ciphergen Biosystems, Inc.
Dr. Michael Boyle
Von Lebig chair in Biomedical Sciences, Juniata College Dept. of Biology
Dr. Tom Lyons Fisher
Professor of Chemistry
Juniata College Dept. of ChemistryAcknowledgements
Altmann F, Kubelka V, Staudacher E, Uhl K, Marz L. 1991. Characterization of the isoforms of Phospholipase A2 from honeybee venom. Insect Biochem 21(5) 467-72.
Kemeny DM, Dalton N, Lawrence AJ, Pearce FL, Vernon CA. 1984. The purification and characterization of hyaluronidase from the venom of the honey bee, Apis Mellifera. Eur J Biochem 139(2) 217-23
Mulfinger LS. 1989. Synergestic activity of honey bee venom with antibiotics. The Pennsylvania State University.
Staay FJ, Fanelli RJ, Blokland A, Schmidt BH. 1999. Behavioral effects of apamin, selective inhibitor of the SKCA channel in mice and rats. Neurosci. Biobehav. Rev. 23 1087-1110