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Laboratory of Microbial Ecology and Technology (LabMET). Research topics and expertise Head : Prof. Dr. ir. Willy Verstraete. Mission . Microbial Ecology. Strategic research. Applied research. Technology. General overview. Strategic research Applied research Tools and instruments

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laboratory of microbial ecology and technology labmet

Laboratory of Microbial Ecology and Technology (LabMET)

Research topics and expertise

Head : Prof. Dr. ir. Willy Verstraete

mission
Mission

Microbial Ecology

Strategic research

Applied research

Technology

general overview
General overview
  • Strategic research
  • Applied research
  • Tools and instruments
  • Focus on SHIME-Tec
strategic research
Strategic Research
  • Microbial communities
  • Quorum sensing
  • Electron shuttling
  • Horizontal gene transfer
  • Metabolomics
applied research
Applied Research
  • Environmental Microbiology
    • Wastewater
      • Microbial fuel cells
      • Biodegradation
      • Anaerobic treatment
      • Nitrogen removal strategies
      • Minimizing wast sludge
applied research6
Applied Research
  • Environmental Microbiology
    • Soil / Sediments
      • Bioprecipitation of catalytic particles
      • Anaerobic removal of organochlorine contaminants
      • Soil and river sludge clean-up
      • Pesticide degradation and ecotoxicology
applied research7
Applied Research
  • Environmental Microbiology
    • Solid Wastes
      • Solid waste treatment
      • De-icing
    • Buildings and structures
      • Biologically mediated CaCO3 formation
      • Microbial induced corrosion
    • Air
      • Indoor air pollution
      • Biotrickling filtration
applied research8
Applied Research
  • Gastro-intestinal Microbiology
    • Functional foods
      • Pro- / pre- / synbiotics
      • Bioactivation of food components
      • Rumen microbiology
    • Risk assessment
      • Environmental contaminants
      • Toxic food processing metabolites
    • Phage therapy
applied research9
Applied Research
  • Foodchain Microbiology
    • Drinking water
      • Hygienisation
      • Water recycling
      • Pathogen abatement
    • Aquaculture systems
  • Habitat research
    • Epiphytes on grain
    • Space station life cycles
    • Deep sea methane oxidation
tools and instruments
Tools and instruments
  • Reactor Technology
    • Simulator of the Human Intestinal Microbial Ecosystem (SHIME)
    • Activated sludge systems
    • Upflow Anaerobic Sludge Bed reactors
    • Microbial Fuel cells
    • Membrane reactors
    • Rotating disc reactors
    • Dialysis reactors
tools and instruments11
Tools and instruments
  • Molecular Analysis
    • PCR
    • DGGE
    • FISH
    • Realtime PCR
    • Cloning
    • Flow Cytometry
tools and instruments12
Tools and instruments
  • Microbial analysis
    • Epifluorescence and light microscopy
    • Growth kinetics
    • Microbial isolations and enrichments
    • Metabolic activity
    • Bioassays
    • Biodegradation assays
tools and instruments13
Tools and instruments
  • Physico – chemical analysis
    • Gas chromatography
    • HPLC
    • Ion chromatography
    • Spectrophotometry
    • Atom absorption
    • BOD, COD, TSS, VSS, NOX, TOC,…
contact information
Contact information

LabMET – Ghent University

Coupure Links 653

B-9000 Gent

http://labMET.ugent.be/

+32/9/264.59.76

microbial conversion of phytoestrogens
Microbial conversion of phytoestrogens
  • Soy phytoestrogens:
    • Daidzine  daidzein  equol (microbial action)
    • Equol has beneficial health effects
    • Microbial consortium applicable as probiotic
  • Hop phytoestrogens:
    • Isoxanthohumol  hoppein (8-prenylnaringenin or 8-PN)
    • Carried out by colon microbiota
    • Importance for hop supplements, beer industry…
colon microbiota activate pahs
Colon microbiota activate PAHs

PAHs are not estrogenic: colon microbiota biotransform

them to estrogenic metabolites

chemopreventive effect from inulin

Beneficial conversion

Short chain fatty acids

Phytoestrogen

Bactericidal compounds

Detrimental conversion

Putrefactive compounds

Toxins

Promutagen  mutagen

(PAH  estrogenic metabolite)

Inulin exerts prebiotic effects

Promotes beneficial conversion

New finding: inulin inhibits conversion of promutagen to mutagen

Chemopreventive effects: added-value property of prebiotics

Chemopreventive effect from inulin

Gut microbiota

decrease of cholesterol levels in blood
Decrease of cholesterol levels in blood
  • Lactobacillus reuteri
  • Bile salt hydrolase activity
  • Reduction of cholesterol levels
  • In vitro:
    • experiments with SHIME indicate survival of probiotic strain under in vitro conditions (stomach, small intestine, colon
  • In vivo:
    • Pigs fed with L. reuteri for 4 weeks showed significantly lower cholesterol levels
    • De Smet et al. 1998. British Journal of Nutrition, 79: 185-194
bioactive peptides

ACE

Bioactive peptides

Aim: are bioactive peptides transported ?

Blood pressure lowering / ACE-inhibitory peptides

ala-leu-pro-met-his-ile-arg (837 g/mol)

Ang I

Ang II

Blood pressure rises

Blood pressure drops

Bradykinin

inactive fragments

phb accumulating bacteria
PHB accumulating bacteria
  • Polyhydroxybutyrate (PHB)
  • Microbial consortium which stores PHB
  • Add consortium as prebiotic to artemia
  • PHB  hydroxybutyrate  butyrate
  • Protection of artemia against pathogens
  • Increased survival
  • Alternative to antibiotics in aquaculture
shime tec29
SHIME-Tec
  • LabMET – Ghent University
  • Coupure Links 653
  • B-9000 Gent
  • www.shimetec.com or .be
  • +32/9/264.59.76