1 / 11

SNAPs and SNAREs

Membrane system- diverse; Transport vesicle system- Selective in nature<br>Transport vesicles : surface markers ( depending on origin & cargo type) that recognizes complementary receptors (Target membrane) <br>2 protein class in control- 1. SNARES (20 types) 2. Rabs ( GTPases targeting) <br>SNARE proteins -central role; i) specificity, ii) bringing the fusion (vesicles with the membrane in target.) Endocytic routes.<br>Exist in supportive sets- 1. v-SNAREs i.e vesicle membrane SNAREs <br> 2. t-SNAREs i.e target membrane SNAREs.

Arked
Download Presentation

SNAPs and SNAREs

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PRESENTATION BY: ARCHITA KEDIA M.Sc LIFE SCIENCES (BIOTECH.) PART II SEMESTER IV PAPER- IV UNIVERSITY OF MUMBAI SNAPs AND SNAREs

  2. MEMBRANE TRANSPORT via SNARE protein and GTPases (Targetting) • Membrane system- diverse; Transport vesicle system- Selective in nature • Transport vesicles : surface markers ( depending on origin & cargo type) that recognizes complementary receptors (Target membrane) • 2 protein class in control- 1. SNARES (20 types) 2. Rabs ( GTPases targeting) • SNARE proteins -central role; i) specificity, ii) bringing the fusion (vesicles with the membrane in target.) Endocytic routes. • Exist in supportive sets- 1. v-SNAREs i.e vesicle membrane SNAREs • 2. t-SNAREs i.e target membrane SNAREs. • T-SNARE (trans), clasp 2 membranes together.

  3. STRUCTURE OF SNARE SNARE motif (cytosolic domain of SNARE segment) comprised of ; • Heptadrepeats (coiled- structures); 60-70 amino acids. • Tightly reversible assembly by V- and t-SNAREs – c/a "trans"-SNARE complexes (formed of 4 -helix bundles). • 3 SNAREs (In synaptic vesicles) : • 1. syntaxin 1 (one helix) • 2. SNAP-25 (2 helix) • 3. Synaptobrevin (VAMP) - ( 1 helix)

  4. SNAREs that're interacting, are separated apart before they can re-enact again through the action of NSF. • Rab proteins - facilitate vesicle docking rate & its regulation, matching of v-SNAREs and t-SNAREs

  5. SNARE HYPOTHESIS • (v-SNAREs) SNARE Vesicle-SNAP-receptors • (t-SNAREs) -Target-SNAP-receptors • SNAP=soluble NSF attachment proteins • NSF=N-ethylmaleimide-sensitive factor

  6. SNAP ( SOLUBLE NSF ATTACHTMENT PROTEINS) • Cytosolic adaptor proteins • Intricated in intracellular transport during membrane vesicular fusion &exocytosis. • Event of vesicle fusion- Assembly and de-assembly. • SNAP + SNARE + NSF = 20S complex. • SNAPs receptor proteins- syntaxin 1, • SNAP-25 (synaptosome associated protein, 25kDa), • and VAMP (synaptobrevin). • Toxicological Significance - blocks transmission- 20S- target neurotoxins, Botulinum

  7. Human SNAP of yeast homologue (Sec17), depicting assembly and disassembly in vesicle fusion membranes with 20 S complex

  8. REFERENCES • Alberts, B., & Wilson, J. (2008). Molecular biology of the cell. Taylor and Francis, Garland. • Snare (protein). Wikiwand. (n.d.). • Wikimedia Foundation. (2022, January 25). Soluble NSF attachment protein. Wikipedia.

  9. THANK YOU FOR LISTENING!

More Related