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Lab Exercise 6a-2. Connective Tissue Nervous Muscle. Classification of connective tissues. Connective Tissue. Connective tissue proper Fluid connective tissue Supportive connecting tissue. Connective tissue proper Loose connective tissue Areolar Adipose Reticular

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lab exercise 6a 2

Lab Exercise 6a-2

Connective Tissue



connective tissue
Connective Tissue
  • Connective tissue proper
  • Fluid connective tissue
  • Supportive connecting tissue
connective tissues
Connective tissue proper

Loose connective tissue




Dense connective tissue

Dense regular

Dense irregular

Elastic tissue

Fluid CT


Supporting CTs


Hyaline cartilage

Elastic cartilage



Connective tissues
areolar tissue
Areolar tissue
  • A loose CTP
areolar what to look for
Areolar: what to look for
  • Fibroblasts
  • Collagen fibers
  • Elastic fibers
  • Mast cells and macrophages
  • Found? Throughout body, under dermis, divides skin from underlying tissues
  • Resting fibroblasts typically have so little cytoplasm that the cells appear, by light microscopy, as "naked" nuclei
adipose tissue
Adipose tissue
  • Another lose CTP (note nucleus)
adipose what to look for
Adipose: what to look for
  • Lots of cytoplasm
  • Slim nuclei pushed off the side
  • Found? You know where
reticular tissue
Reticular tissue
  • The third type of loose CTP
reticular what to look for
Reticular: what to look for
  • Reticular fibers (network)
  • Found? Internal framework in many sort organs (liver, spleen) supporting the parenchyma
dense ctp
Dense CTP
  • Dense regular – strength in one direction
  • Dense irregular – strength in all directions
  • Elastic tissue - pliable
dense regular what to look for
Dense regular: what to look for
  • Thick parallel bundles of collagen
  • Small fibroblasts in between bundles
  • Found? Tendons, ligaments, deep fascia.
dense irregular what to look for
Dense irregular: what to look for
  • Mesh of collagen fibers (irregular looking)
  • Interspersed fibroblasts
  • Found? Dermis of skin, periosteum, perichondrium
elastic tissue what to look for
Elastic tissue: what to look for
  • Elastic fibers (instead of collagen fibers) in large bundles
  • Fibroblasts
  • Found? Between vertebrae, in blood vessel walls (underneath endothelium)
blood what to look for
Blood: what to look for
  • RBCs
  • White blood cells (darker): monocytes, lymphocytes, granulocytes
  • Platelets
supportive ct
Supportive CT
  • Cartilage – gelatinous, padding
    • Hyaline cartilage
    • Elastic cartilage
    • Fibrocartilage
hyaline cartilage
Hyaline cartilage
  • Glasslike because fibers not visible
more hyaline
More hyaline
  • There are collagenous and elastic fibers lying in the cartilage matrix but they are invisible because their “refractive index” is the same as that of the matrix (like cornea)
  • Hyaline cartilage (lavender matrix), with perichondrium (pink) outside it. The latter is a dense regular collagenous CT. Cartilage cells = chondrocytes, and they are lying in the lacunae.
hyaline cart what to look for
Hyaline cart.: what to look for
  • Chondrocytes and lacunae
  • No visible fibers
  • Where? Most joints, nasal septum
elastic cart what to look for
Elastic cart: what to look for
  • Many elastic fibers in matrix
  • Chondrocytes in lacunae
  • May be stacked up
fibrocartilage what to look for
Fibrocartilage: what to look for
  • Irregular, wispy collagen fibers
  • Chondrocytes
  • Found? Intervertabral discs of spine, pads in knee joint
Detail of lacuna, showing radiating canaliculi. Tissue fluid from the capillaries and connective tissue of the Haversian canal can seep through these spaces and channels, bringing nutrients to the stellate osteocytes residing there.
bone what to look for
Bone: what to look for
  • Osteon (whole circular structure)
  • Concentric lamellae (of matrix)
  • Central canal (at center of lamellae)
  • Osteoblasts
  • Osteocytes in lacunae
  • Canaliculi

Found? Bones!

nervous tissue
Nervous tissue
  • Neuron smear
  • Large, pyramidal cell bodies
  • Long processes extending out
nervous tissue41
Nervous Tissue

Figure 4.10

3 types of muscle tissue
3 Types of Muscle Tissue
  • Skeletal muscle:
    • large body muscles responsible for movement
  • Cardiac muscle:
    • found only in the heart
  • Smooth muscle:
    • found in walls of hollow, contracting organs (blood vessels; urinary bladder; respiratory, digestive and reproductive tracts)
muscle tissue skeletal
Muscle Tissue: Skeletal
  • Long, cylindrical, multinucleate cells with obvious striations
  • Found in skeletal muscles that attach to bones or skin
muscle tissue cardiac
Muscle Tissue: Cardiac
  • Branching, striated, uninucleate cells interlocking at intercalated discs
muscle tissue smooth
Muscle Tissue: Smooth
  • Sheets of spindle-shaped cells with central nuclei that have no striations
  • Found in the walls of hollow organs
  • Look at all slides
  • Draw an example of each tissue on paper provided
  • 11 connective tissues:
    • 6 CTP (3 loose, 3 dense)
    • 1 Fluid CT (blood)
    • 4 Supportive CT (3 cartilage, 1 bone)
  • Neurons
  • 3 Muscle tissues
    • Skeletal
    • Striated
    • Smooth
turn in on thurs 10 25
Turn in on Thurs 10/25
  • 7 drawings from 6a-1 Epithelia
  • 15 Drawings from 6a-2 Connective+
  • Review sheet for lab 6a