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Chiba iGEM 2007. Marimo. What the hell is Marimo?. Marimo (Japanese: 毬藻 marimo ), also known as Cladophora ball, Lake ball, or Moss Balls in English, is a species of filamentous green algae ( Chlorophyta ) found in a number of lakes in the northern hemisphere. blabla…. 1. 2. 3.

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What the hell is marimo
What the hell is Marimo?

  • Marimo (Japanese: 毬藻marimo), also known as Cladophora ball,Lake ball, or Moss Balls in English, is a species of filamentous green algae (Chlorophyta) found in a number of lakes in the northern hemisphere.

  • blabla…


To make a bacterial marimo

まりも完成

To make a bacterial Marimo


Our system
Our System

  • Gather bacteria

    • Quorum Sensing

  • Stick bacteria together

    • flagella: fliC gene

  • Control size (advanced)

    • Tune Quorum Sensing


Detailed design

AHL

AHL

AHL

AHL

Detailed Design


Detailed design sender cell
Detailed Design: Sender Cell

  • Constitutively expressflagella

  • Produce AHL


Detailed design reciever cell
Detailed Design: Reciever Cell

  • LuxP > flagella

  • no AHL > aiiA ??


Detailed design overview

AHL

AHL

AHL

AHL

Detailed Design: Overview

①Senderは常に鞭毛が生えていて、AHLを生産する。

②ReceiverはAHL存在下で鞭毛が生えて、AiiAによりAHLを分解する。

③鞭毛にはヒスタグが付いているのでニッケルイオンを仲介としてくっつく。


Experiments results

Experiments & Results

Quorum sensing, FliC-his gene


Experiments
Experiments

  • Quorum Sensing

    • modify? for a lab-class 自由溶液

    • size-tunable

  • Flagella (fliC) engeneering


Quorum sensing in a liquid
Quorum Sensing in a liquid…

  • To make the AHL conc. gradation

    • Sender(=AHL producer) Problem

      • Senderを固める(拡散させない)

      • 必要量のAHLを出すSenderの数

    • AHL拡散 Problem

      • solvent’s viscosity

      • AHL溜る -> 分解する(aiiA)


Size control
Size Control

  • Super Sender Cell

    • Produce more AHL

  • Super Reciever Cell

    • Sensitive


To make a marimo
To Make a Marimo

  • Check the flagella adsorption w/Quorum sensing

  • 2-dimensional test @ plate

  • 3-dimensional (marimo)

  • Size control test





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