Genetic Engineering. 4.4.1. Brief Summary of the Unit.
Students will be able
PCR was developed in 1983 by KaryMulis who received the Nobel prize for chemistry in 1993 for his work.
PCR is carried out in a thermal cycler
Gel electrophoresis can separate nucleic acids that differ in size. DNA fragments created by restriction enzymes are separated based on their rate of movement through a gel in an electric field.
How far a DNA molecule travels is inversely proportional to its length.
After the current is turned off, a dye is added; this reveals the separated bands by fluorescing in ultraviolet light.
DNA profiling to determine paternity uses the concept that all of the child’s DNA comes from its parents. Each band shown on the DNA profile of a a child must correspond with a band in the profile of the father or mother.
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