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Tritiated (3H) thymidine PowerPoint PPT Presentation


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3 H-thymidine. Tritiated (3H) thymidine. 3 H-thymidine. Original method for measuring cell proliferation Radioactive Not compatible for multiplexed analyses. Br. Br. Br. Br. BrdU. BrdU (5-bromo-2 ' -deoxyuridine). Br. Br. Br. Br. BrdU. Acid or DNase. Br. Br. Br. Br. BrdU.

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Tritiated (3H) thymidine

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3H-thymidine

Tritiated (3H) thymidine


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3H-thymidine

  • Original method for measuring cell proliferation

  • Radioactive

  • Not compatible for multiplexed analyses


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Br

Br

Br

Br

BrdU

BrdU (5-bromo-2'-deoxyuridine)


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Br

Br

Br

Br

BrdU


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Acid or DNase

Br

Br

Br

Br

BrdU


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Br

Br

Br

Br

BrdU


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Br

Br

Br

Br

BrdU

  • Non-radioactive

  • Multiplex compatible but, strand separation requirement for anti-BrdU access, can affect:

    • Ability for other antibodies to bind

    • Morphology

    • Ability for dyes that require dsDNA to bind efficiently, i.e., cell cycle dyes


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Click-iT™ EdU

EdU (5-ethynyl-2'-deoxyuridine)


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Click-iT™ EdU


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Click-iT™ Edu

  • Non-radioactive

  • No DNA denaturation required

  • Simplified protocol

  • Small molecule detection

  • Multiplex compatible, including

    • Other antibodies

    • Dyes for cell cycle analysis


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