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Nanoengineering of neuronal growth substrates. Complex architecture of vertical and lateral micropatterns Polylysine, gelatin, Phospholipase A, or BSA. Template used for LBL-LO fabrication process. (a), (b)photolithography, (c) LbL assembly process, (d) LbL processed chips,

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nanoengineering of neuronal growth substrates
Nanoengineering of neuronal growth substrates
  • Complex architecture of vertical and lateral micropatterns
  • Polylysine, gelatin, Phospholipase A, or BSA
template used for lbl lo fabrication process
Template used for LBL-LO fabrication process
  • (a), (b)photolithography, (c) LbL assembly process, (d) LbL processed chips,
  • (e) after lift-off, (f) top view of the fabricated chip: green-FITC labeled sPLA2, red-TRITC labeled BSA/PLL/gelatin.
confocal scanning shows separate dye regions selective patterning of peptides
Confocal scanning shows separate dye regions: selective patterning of peptides

Biomacromolecules: 5: 1745, 2004.

nanoscale neuro coatings
Nanoscale neuro coatings
  • LbL layer by layer PEI+/Ln- onto Si. “Polyion glue”
  • Coatings need isoelectric pt. Away from neutral pH.
  • QCM to weigh layer, ELISA to assay Ln sites
  • Impedance spectroscopy
  • Reduce scarring & fibro-encapsulation?.
  • PEI is linear, CH is bulky

uncoated

[PEI-Ln]8

He, W. Biomaterials 26: 2983, 2005

hearing sensitivity
Hearing sensitivity?
  • 100 hairs in a hair cell (inner) transduce bending
  • Outer hair cells are silent majority that bend actively for amplification of motion
  • High Speed of OHCs ?
  • Electromotility of OHCs – prestin: BW > 100 kHz (in vitro), but Cell membrane impedance filters @ 1Khz. I.e. time constant of V response….
pushing on ohc with piezo
Pushing on OHC with piezo

Kennedy et al. Nature, 2005

mech propertis of ohc
Mech Propertis of OHC
  • Negative stiffness = force generation
force generation by ohc
Force generation by OHC

Kennedy et al. Nature, 2005

boltzman model
Boltzman model
  • where P\'s are the probabilities of states or classes 1 and 2, and DF is free energy. For example Pi can represent the concentration of particles in 2 separate compartments. Lets say the energy in the first compartment is F1 and in the second, it is F2, and DF= F2-F1. Since the particles must either be in compartment 1 or 2, then P1 + P2 =1.
slide13
DPOAE level Ldp measured for EMF-exposure, i.e., within transmission pauses ~rhombuses!, and for sham exposure ~filled circles!, for one individual subject. Open circles indicate
  • the corresponding noise-floor level Lnoise . There were 12 different
  • tests where frequency f 2 and level L2 of the primary tones were varied. f 2
  • and L2 are indicated at the respective panels ~A!–~L!. Chosen case is representative
  • of the 24 subjects in whom the EMF-correlated change in DPOAE
  • level was smaller than 0.25
measuring cochlear function
Measuring cochlear function
  • Distortion product otoacoustic emissions
  • Two tones given to ear: 2f1-f2 = best DPOAE
  • Most sensitive assay of cochlear function
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