Figure 4: The duration of the first six calls emitted after each type of startle pulse Was unchanged between day sixteen and nineteen in saline (n=8) pre-treated animals Was significantly shorter in the PCP (n=7) group (n=7)
For Figure 1 to 3, all data are presented as means ± SEM and analysed using a two-way ANOVA with post hoc Bonferroni tests when significance reached 95%.P < 0.01 (**)
Figure 1: The time course in days of the experiment.
The percentage of prepulse inhibition was calculated as:
The effects of chronic phencyclidine treatment on prepulse inhibition in rats; ultrasonic vocalisations reveal fear conditioning Beth Tunstall, Dr Simon Beckett and Dr Rob Mason
School of Biomedical Sciences, Queen’s Medical Centre, Nottingham, UK
Male Sprague-Dawley rats were treated once a day for 14 days with either PCP (5mg.kg-1 i.p., n=10) or saline (1ml.kg-1i.p., n=10) from 30 days old. Acoustic startle response (ASR) was tested on day 0, and PPI on day 16 and 19 (Figure 1). USVs were recorded during testing with an Ultrasound Gate (Customised model 112, Avisoft, DE), and analysed post hoc (v 4.38 SASLab-Pro, Avisoft, DE). Statistical analyses were carried out using Prism (v 4 Graphpad, USA). PPI data were analysed using a Two-way ANOVA with Bonferroni post hoc tests when significance reached 95%.