HILL LAB RESEARCH Metabolomics by MALDI-IMMS. OVERVIEW. MALDI-IMS-oTOF. CONCLUSIONS. Purpose: Two dimensional analysis of intracellular metabolites in Escherichia coli (E. coli) . Method: E. coli metabolites were analyzed by MALDI-MS and MALDI-IMS-oTOF MS.
Metabolomics by MALDI-IMMS
Purpose:Two dimensional analysis of intracellular metabolites in Escherichia coli (E. coli).
Method:E. coli metabolites were analyzed by MALDI-MS and MALDI-IMS-oTOF MS.
Results: E. coli metabolites were resolved using MALDI IMS-MS.
This project utilizes the novel approach for the separation and detection of metabolites in a complex biological system, such as E.coli, by using a MALDI-IMS-oTOF MS. The MALDI-IMS-oTOF MS has the ability to resolve the metabolite peaks from the background along with simultaneously separating metabolite isomers. The MALDI-IMS-oTOF MS is a promising method for determination of the metabolome in complex biological systems.
E. coli metabolites were extracted using methanol and spiked with ribitol as an internal standard.
2,5-dihydroxybenzoicacid was the MALDI matrix.
A Voyager DE-RP MALDI-TOF mass spectrometer instrument at Washington State University was used for the MALDI-MS experiments.
The MALDI-IM-oTOF system and its supporting electronics were designed and constructed at Ionwerks Inc. (Houston, TX).
This work was supported in part by a grant from the National Institute of Health-Road Map Grant #DK070274