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S- Sepharose C-101. Andy Hunter Alec Mackenzie Trevor Seelert. Purpose of Unit. Separate Proinsulin and other proteins from basic impurities Prepare the stream for further downstream purification. Cation exchange Chromatography. Equilibrate Load Rinse Elute Wash Regenerate.

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S sepharose c 101

S-SepharoseC-101

Andy Hunter

Alec Mackenzie

Trevor Seelert


Purpose of unit
Purpose of Unit

  • Separate Proinsulin and other proteins from basic impurities

  • Prepare the stream for further downstream purification


Cation exchange chromatography
Cation exchange Chromatography

  • Equilibrate

  • Load

  • Rinse

  • Elute

  • Wash

  • Regenerate



Process flow sheet1
Process Flow Sheet

New

Design


Process flow sheet2
Process Flow Sheet

New

Design


S sepharose design
S-Sepharose Design

  • Scale-up keeping constant values


S sepharose design1
S-Sepharose Design

  • Scaled-up from US 101013 Patent


S sepharose design2
S-Sepharose Design

  • GE Chromaflow

  • GE AxiChrom


S sepharose design3
S-Sepharose Design

  • GE Chromaflow Column

  • Currently max D = 1200mm, H = 30cm

  • Custom Design available for D > 1200mm


Economics and suppliers
Economics and Suppliers

  • S-Sepharose available from GE Healthcare

  • Chromaflow supplied by GE


Iec alternatives
IEC Alternatives

  • SP-Sepharose: Sulphopropyl

    • –O–CH2CHOHCH2OCH2CH2CH2SO3–

  • S-Sepharose: Sulphoethyl

    • no longer produced

    • –O–CH2–CH2–SO3–

  • Replaced by SP-Sepharose

  • Anion exchange resin

    • Q-sepharose

    • DEAE

  • HIC Columns

  • SEC/Gel Filtration


  • Iec alternatives1
    IEC Alternatives

    • Comparison of IEC


    No questions allowed
    No Questions Allowed

    Disclaimer: The views presented in this slide are not necessarily those of any or all team members.



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