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S- Sepharose C-101. Andy Hunter Alec Mackenzie Trevor Seelert. Purpose of Unit. Separate Proinsulin and other proteins from basic impurities Prepare the stream for further downstream purification. Cation exchange Chromatography. Equilibrate Load Rinse Elute Wash Regenerate.

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S- Sepharose C-101

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S-SepharoseC-101

Andy Hunter

Alec Mackenzie

Trevor Seelert


Purpose of Unit

  • Separate Proinsulin and other proteins from basic impurities

  • Prepare the stream for further downstream purification


Cation exchange Chromatography

  • Equilibrate

  • Load

  • Rinse

  • Elute

  • Wash

  • Regenerate


Process Flow Sheet


Process Flow Sheet

New

Design


Process Flow Sheet

New

Design


S-Sepharose Design

  • Scale-up keeping constant values


S-Sepharose Design

  • Scaled-up from US 101013 Patent


S-Sepharose Design

  • GE Chromaflow

  • GE AxiChrom


S-Sepharose Design

  • GE Chromaflow Column

  • Currently max D = 1200mm, H = 30cm

  • Custom Design available for D > 1200mm


Economics and Suppliers

  • S-Sepharose available from GE Healthcare

  • Chromaflow supplied by GE


IEC Alternatives

  • SP-Sepharose: Sulphopropyl

    • –O–CH2CHOHCH2OCH2CH2CH2SO3–

  • S-Sepharose: Sulphoethyl

    • no longer produced

    • –O–CH2–CH2–SO3–

  • Replaced by SP-Sepharose

  • Anion exchange resin

    • Q-sepharose

    • DEAE

  • HIC Columns

  • SEC/Gel Filtration


  • IEC Alternatives

    • Comparison of IEC


    No Questions Allowed

    Disclaimer: The views presented in this slide are not necessarily those of any or all team members.


    Just Kidding!


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