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iGEM

iGEM. Advisors Meeting 4/23/2010. First week of summer Project Proposal Where to start research on our project? Biosafety ! Regional Conference Update Who is interested in going to the Competition November 5th-7th? Access to IGB. Summer Meetings. Tools + Wetlab general stuff– 1/wk

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iGEM

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  1. iGEM Advisors Meeting 4/23/2010

  2. First week of summer • Project Proposal • Where to start research on our project? • Biosafety! • Regional Conference Update • Who is interested in going to the Competition November 5th-7th? • Access to IGB

  3. Summer Meetings • Tools + Wetlab general stuff– 1/wk • Tools + Wetlab Administrative stuff – 1/wk • Wetlab + Professors – 1/wk • Wetlab – 1/day 10am (except May 23-27th)

  4. May 23rd – 27th Mornings Afternoon – Lab Work! Pull Reporters/Promoters/etc. from Parts Registry Standard Assembly Basic modeling Testing Work on Project Procedure • Day 1 • Procedure for Intro Project • Day 2 • How to research • Parts Registry • Primer Design • Day 3 • OpenWetWare/Wiki • Modeling Basics • Day 4 • Data Collection • Bioethics • Day 5 • Meetings, etc.

  5. Project Proposal • Use pre-existing pathways to make e. coli. Take in metal from the surroundings and reduce it to precipitate metal ores. • Focus on two different metals and an alloy of the two • Create and a decoder function • Using tf, RNA regulation, or both? • Combine the decoder and metal reducing pathways to make a cell that will produce either one or the other type of metal, or an alloy when the two are both present.

  6. Project Proposal • The 2010 Illinois iGEMBioware team project involves the construction of a biological system that functions as a decoding nanofabricator. As a continuation of the previous Illinois iGEMBioware project: The Bacterial Decoder, the decoding bionanofabricator will produce specifically structured, purified, metallic ores depending on the presence of metals in its environment. This system will also possess the capacity to produce metal alloys by analyzing the presence and combination of specific metals in the environment. This system will take advantage of metal-respiring and metal detoxifying microbes that possess the ability to reduce metals as terminal electron acceptors and/or reduce toxic forms of metals into insoluble, harmless forms. The decoding nanofabricator will emphasize the capacity of information processing in biological systems to perform human-define tasks in industrial, manufacturing, computing, and environmental applications.

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