Introduction to the oil immersion compound microscope. Purpose of microscope. Microscopy is the technology of making very small things visible to the human eye. Used in the diagnosis of many diseases. Used for soil, insects and rocks. Used in pathological laboratories and medical.
Its includes three parts:
It consists of the following parts:
To control the distance between the ocular lenses to adapt the distance between viewers eyes so, the eyepiece lenses will spread apart or get closer together to fit each individual.
Microscope has two sets of lenses are :
Powers of theeyepiece (10X) multiplied by objective lenses determine total magnification.
sees 0.2 um
1 mm=10-3 m
1 um=10-6 m
1 nm=10-9 m
Numerical Aperture (N.A.): This is a number that expresses the ability of a lens to resolve fine detail in an object being observed. It is derived by a complex mathematical formula and is related to the angular aperture of the lens and the index of refraction of the medium found between the lens and the specimen.
Lighting system consists of modern microscopes of the following parts :
controls the amount of light allowed to pass through the specimen
Always carry a microscope with one hand holding the arm and one hand under the base.