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  1. Summary of the sequence analysis of catrocollastatin-C,a 23.6-kD protein found in the venom of the western diamondback rattlesnake Crotalus atrox.Sequences shown are given in the one-letter amino acid code.The overall amino acid sequence(216 amino acid residues long)for catrocollastatin-C as deduced from the overlapping sequences of peptide fragments is shown on the lines headed CAT-C.The other lines report the various sequences used to obtain the overlaps.These sequences were obtained from(a)N-term:Edman degradation of the intact protein in an automated Edman sequenator;(b)M:proteolytic fragments generated by CNBr cleavage,followed by Edman sequencing of the individual fragments(numbers denote fragments M1through M5);(c)K:proteolytic fragments(K3 through K6)from endopeptidase Lys-C cleavage,followed by Edman sequencing;(d)E:proteolytic fragments from Staphylococcus protease(E13 through E15)digestion of catrocollastatin sequenced in the Edman sequenator.

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