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A field and laboratory exercise that studies the relationship between Dalmation toadflax and its biological control agent, the stem weevil. Field data collection. a) estimate stem density of toadflax at one or more sites. b) collect a sample of stems of toadflax from one or more sites

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A field and laboratory exercise that studies the relationship between Dalmation

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A field and laboratory exercise that studies the relationship between dalmation

A field and laboratory exercise that studies the relationship between Dalmation

toadflax and its biological control agent, the stem weevil.

Field data collection.

a) estimate stem density of toadflax at one or more sites.

b) collect a sample of stems of toadflax from one or more sites

Lab work.

1. Carefully measure 20 cm sections of toadflax stems (note: if lots of egg scars,

use only 10 cm. Cut the stems to this length.

Count egg scars (see 4th PPT slide) per unit of stem.

Carefully dissect the stem and see how many weevils you can find.

Class effort:

Graph class results: numbers of weevils found versus number of egg scars.

Compare your egg scar average to that found on the Front Range, 2010-

2013. How does your site compare.

3) Based on your numbers and graph of relationship between egg scars and

plant reproduction (5th PPT slide), how healthy is your population of

toadflax?


Toadflax life cycle

Toadflax Life Cycle

5 cm

1 mm

Seed

Seedling

Adult

Rosette


Interaction weevils and toadflax

Interaction: Weevils and Toadflax

Egg

5 cm

Weevils develop in the stems. Can the weevil control the plant?

~5 mm

1 mm

~5 mm

Seed

Larva

Seedling

Adult

Adult

~5 mm

Rosette

Pupa


A field and laboratory exercise that studies the relationship between dalmation

The small pinholes in the toadflax stems are egg scars (sites where

females laid their eggs that subsequently

burrowed into the stem.)

The larger holes are where adult weevils likely

emerged(this stem was collected in late August, two months

after egg-laying)


A field and laboratory exercise that studies the relationship between dalmation

Eggs laid per centimeter of stem on Dalmation toadflax

(results from five sites found along Colorado Front Range)

Egg scars per cm of stem

2011 2012 2013

Year


A field and laboratory exercise that studies the relationship between dalmation

Effects of stem weevils on plant reproduction

Flowers or Pods per Stem

Number of egg scars per cm of stem


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