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ThermoTRP channels expression in the broodpatch of junglefowl during egg incubation

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ThermoTRP channels expression in the broodpatch of junglefowl during egg incubation - PowerPoint PPT Presentation


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ThermoTRP channels expression in the broodpatch of junglefowl during egg incubation. The regulation of egg temperature requires the transfer of heat from the brood patch skin and regulation of broodpatch blood flow The broodpatch needs:

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slide1

ThermoTRP channels expression in the broodpatch of junglefowl

during egg incubation

  • The regulation of egg temperature requires the transfer of heat from the brood patch skin and regulation of broodpatch blood flow
  • The broodpatch needs:
    • The presence of thermo receptors namely thermoTRPs
    • An appropriate vasomotor response and morphological change
  • We tried to detect :
    • The presence of thermoTRP channels (V1, V3, V4, A1 and M8) in different junglefowl tissues
    • Changes of brood patch: Regulation in TRP channels?
    • Morphological changes?
slide2

M8 in all checked tissues (Heart, Kidney, Liver, DRG, Blood, WBC and SKIN)

  • V1 in all except Blood
  • V3 in all except Blood and WBC
  • A1 in None except Liver and DRG
slide3

M8 in all checked tissues (Heart, Kidney, Liver, DRG, Blood, WBC and SKIN

  • V1 in all except Blood
  • V3 in all except Blood and WBC
  • A1 in None except Liver and DRG

Only Genomic fragments for V4

slide4

M8 in all checked tissues (Heart, Kidney, Liver, DRG, Blood, WBC and SKIN

  • V1 in all except Blood
  • V3 in all except Blood and WBC
  • A1 in None except Liver and DRG

In the skin

Only Genomic fragments for V4

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  • No change in V1 & V3 expression in qPCR!
  • M8?

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slide9

thermoTRP’s expression in junglefowl ‘s tissues

  • TRPA1 not in the skin. Why?
  • TRPV4 mRNA was not found in any tissue. Why?
  • Not expressed?
  • Alternative splicing?
  • Brood patch morphological changes
  • Epidermal increase, AVA’s frequency amplification, AVA’s area growth
  • TRP channels regulation in Epidermis?
  • Or AVAs? Or in the earlier stages?
slide10

Although we can see considerable morphological changes in the skin of broodpatch the tested thermoTRP channel’s expression did not show significant change

  • TRP channels are so specific to some parts of skin?
  • TRP channels regulation is only in AVAs or epidermis? Or in first stages of incubation?
  • V4 a mystery!
  • Thank you!
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