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A. BalI. /. BsaAI. HindIII. MscI. XhoI. AvaI. +888. +928. +960. +1019. +1041. Hind III - AvaI. HindIII - MscI. HindIII - XhoI. HindIII - BsaI. B. kDa. 97. p90. 69. 53. 1. 2. 3. 4. 5. C AGCTTTATTA TTCAAGGCTA TACGCAGGGA TCAGCAGCCT

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+1041

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1041

A

BalI

/

BsaAI

HindIII

MscI

XhoI

AvaI

+888

+928

+960

+1019

+1041

Hind III - AvaI

HindIII - MscI

HindIII - XhoI

HindIII - BsaI

B

kDa

97

p90

69

53

1

2

3

4

5

C

AGCTTTATTA TTCAAGGCTA TACGCAGGGA TCAGCAGCCT

TTGGCCATTG CAGAAGGAAA CACCCAGAAG GCTCGAGATA

AGTGATGCAG AGAATCACAG TTCCACAGCA ACCCGGAGAC

TGCTTGTTCC ACGTATAAGG CTTATATTTT TC


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