Dr. Paul Gollnick
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Dr. Paul Gollnick. Regulation of the tryptophan genes in Bacillus subtilis. TRAP. t rp R NA-binding A ttenuation P rotein. - tryptophan. + tryptophan. TRAP does not. TRAP is Activated. Bind RNA. to Bind RNA. trp genes are Expressed. trp genes are Repressed.

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TRAP

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Trap

Dr. Paul Gollnick

Regulation of the tryptophan genes in Bacillus subtilis

TRAP

trpRNA-binding Attenuation Protein

-tryptophan

+tryptophan

TRAP doesnot

TRAP isActivated

Bind RNA

toBind RNA

trp genes areExpressed

trp genes areRepressed


Trap

Attenuation control of the B. subtilis trp operon


Trap

The Structure of TRAP

Antson et al. Nature 374; 693-700, 1995


Trap

The Structure of TRAP bound to RNA

Antson et al. Nature 401; 235-242 1999


Trap

Crystal structure of TRAP complexed with (GAGAU)11

Antson et al. Nature 401; 235-242 1999


Trap

Attenuation control of the B. subtilis trp operon


Trap

TRAP Binds to RNA by a 5’ to 3’ Directional Mechanism

3’

GAG

GAG

GAG

GAG

GAG

GAG

GAG

5’

5’

GAG

GAG

GAG

GAG

3’


Trap

TRAP binding induces RNA cleavage


Trap

Attenuation control of the B. subtilis trp operon


Trap

TRAP-RNA

TRAP-RNA

RNA

RNA

Anti-TRAP prevents TRAP from binding RNA

AT added prior to RNA

AT

KD = 10.1 μM

RNA added prior to AT

AT

Yanling Chen


Trap

Crystal structure of Anti-TRAP

Mikhail Shevtsov and Alfred Antson


Trap

Nonsense Mediated Decay (NMD)

Several Proteins involved in degrading

mRNA Contain PIN Domain


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