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Dr. Paul Gollnick. Regulation of the tryptophan genes in Bacillus subtilis. TRAP. t rp R NA-binding A ttenuation P rotein. - tryptophan. + tryptophan. TRAP does not. TRAP is Activated. Bind RNA. to Bind RNA. trp genes are Expressed. trp genes are Repressed.

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Presentation Transcript
slide1

Dr. Paul Gollnick

Regulation of the tryptophan genes in Bacillus subtilis

TRAP

trpRNA-binding Attenuation Protein

-tryptophan

+tryptophan

TRAP doesnot

TRAP isActivated

Bind RNA

toBind RNA

trp genes areExpressed

trp genes areRepressed

slide3

The Structure of TRAP

Antson et al. Nature 374; 693-700, 1995

slide4

The Structure of TRAP bound to RNA

Antson et al. Nature 401; 235-242 1999

slide5

Crystal structure of TRAP complexed with (GAGAU)11

Antson et al. Nature 401; 235-242 1999

slide8

TRAP Binds to RNA by a 5’ to 3’ Directional Mechanism

3’

GAG

GAG

GAG

GAG

GAG

GAG

GAG

5’

5’

GAG

GAG

GAG

GAG

3’

slide11

TRAP-RNA

TRAP-RNA

RNA

RNA

Anti-TRAP prevents TRAP from binding RNA

AT added prior to RNA

AT

KD = 10.1 μM

RNA added prior to AT

AT

Yanling Chen

slide12

Crystal structure of Anti-TRAP

Mikhail Shevtsov and Alfred Antson

slide13

Nonsense Mediated Decay (NMD)

Several Proteins involved in degrading

mRNA Contain PIN Domain

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