Varying Alcohol Concentrations The Rats Drink
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Varying Alcohol Concentrations The Rats Drink Nasrin Akter Dr. Michael Lewis Hunter College Harlem Children Society. Introduction. Scientists use animals when the use of humans is either impractical or unethical.

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Introduction 3529558

Varying Alcohol Concentrations The Rats DrinkNasrin AkterDr. Michael LewisHunter CollegeHarlem Children Society


Introduction

Introduction

  • Scientists use animals when the use of humans iseither impractical or unethical.

  • For example when scientists investigate fetal alcohol syndrome, they cannot give alcohol to pregnant women but they can give to pregnant animals such as rats.

  • Scientific research with animals has contributed to many important advances in scientific & medical knowledge.


Intro cont

Intro Cont

  • .Mice and rats are a useful model for studying the effects of alcohol.

  • The SD rats are more active during the night then during the day.

  • SD rat is a general model for the study of human health and disease.

  • The SD rats is a widely accepted, dependable, general purpose research model used in virtually all disciplines of biomedical research including toxicology and pharmacology.


Http www hku hk launit pictures sc rat jpg

http://www.hku.hk/launit/pictures/sc_rat.jpg


Materials

Materials

  • Sprague Dawley (SD) rats

  • 1% concentration=10ml EtOH, 990ml H2O

  • 3% concentration=30ml EtOH, 970ml H2O

  • 5% concentration=50ml EtOH, 950ml H2O

  • 7% concentration= 70ml EtOH, 920ml H2O

  • 9% concentration=100ml EtOH,910ml H2O


Introduction 3529558

ithttp://www-personal.umich.edu/~nbutler/ninab7.jpg


Methods

Methods

  • We feed EtOH to SD rats once a week. We weight the bottles into grams (g) before we feed them and also after we feed them.

  • Record the time we place the bottles on the cage.

  • Wait exactly one hour before we remove the bottles.

  • Each rat has its own separate EtOH and H2O bottle.

  • There is 22 SD rats, Each bottle is numbered 1-22 for rat 1-22 there was sign that was set up for the bottle.

  • Bottles with orange tape are for EtOh and those with blue are for water

    (rats#1 gets orange#1 and blue #1)


Que tion

Que$tion

  • Do SD rats drink more alcohol (EtOH) than water (H20), or more water (H20) than alcohol (EtOH)?


Introduction 3529558

Average Alcohol and Water

the Rats Drank

6

5

4

Alcohol

Amount (g)

3

Water

2

1

0

0

2

4

6

8

10

Concentration (%)


References

References

  • http://www.personal.umich.edu/~nbutler/ninab7.jpg

  • http://www.hku.hk/launit/pictures/sc_rat.jpg

  • http://science.education.nih.gov/supplements/nih3/alcohol/guide/info-alcohol.htm

  • http://www.liq.wa.gov/PriceBook/bac_page.asp

  • http://www.franklin.uga.edu/casar/Training/rat%20training/Biomethodology%20of%20the%20Rat.htm#Behavior


Acknowledgments

Acknowledgments

  • Dr. Michael Lewis

  • Dr. Sat Bhattacharya

  • Micki

  • Daisy

  • Harlem children society

  • Hunter College

  • M.S.K.C.C


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