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Figure S1. Lee et al. 16. LL-37. A B . 12. 8. 4. Mabc. Relative mRNA expression. DEFB4. 20. (h) 0 3 6 18 48. 15. LL-37. 10. 5. DEFB4. 0.  -actin. 0 3 6 18 48 (h). Mabc. 20. IL-1 . IC. α -Dectin-1. 15. U M U M. 10.

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Figure S1. Lee et al.

16

LL-37

A

B

12

8

4

Mabc

Relative mRNA expression

DEFB4

20

(h) 0 3 6 18 48

15

LL-37

10

5

DEFB4

0

-actin

0 3 6 18 48 (h)

Mabc

20

IL-1

IC

α-Dectin-1

15

U M U M

10

IL-1

5

LL-37

LL-37

16

DEFB4

12

Relative mRNA expression

-actin

8

4

20

DEFB4

15

10

5

0

U M

U M

α-Dectin-1

IC


8

Casp1 p10/

-actin

6

SN

Cell

4

2

50 kD

Figure S2. Lee et al.

35 kD

8

35 kD

6

IL-1 p17/

-actin

Mabc

4

24 kD

2

PIC

USC

0

USC

PIC

Casp1

A

B

Mabc

pro-IL-1

-actin

Casp1 p10

15 kD

IL-1 p17

7 kD

20 kD

15 kD

1600

1200

800

TNF-α (pg/ml)

400

0

USC

PIC

Mabc


Figure S3. Lee et al.

A B

C

SN

Cell

50 kD

280

Mabc

35 kD

sip62

siNS

None

210

(h) 0 3 6 18 48

35 kD

p62

p62

IL-1 (ng/ml)

140

24 kD

***

-actin

-actin

70

0

U M

U M

UM

siNS

sip62

sip62

siNS

U M

U M

U M

Casp1

pro-IL-1

8

Casp1 p10/

-actin

6

15 kD

-actin

4

7 kD

2

Casp1 p10

8

6

IL-1 p17/

-actin

20 kD

4

IL-1 p17

2

15 kD

0

U M

U M

U M

siNS

sip62


Figure S4. Lee et al.

shNS

siNS

sip62

shp62

120

sip62

80

Cell viability (%)

40

0

120

shp62

80

40

0

0 6 12 24 (h)


Figure S5. Lee et al.

A

B

C

ATP

nigericin

(h) 0 3 6 18 48

0 3 6 18 48 (h)

p62

-actin

shNS

shp62

UANUANUAN

IL-1β

p62

-actin

280

210

140

IL-1 (ng/ml)

70

0

U A N

U A N

U A N

shNS

shp62


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