html5-img
1 / 12

Mantoux – in vivo detection of specific cell immunity after exposition to antigen

Mantoux – in vivo detection of specific cell immunity after exposition to antigen - burden of patient by antigen - possible immunodeficiency of patients (anergy, risk of alergic reaction) - memory cell after BCG vaccination, - exposition to antigen = activation of MC

xyla-cantu
Download Presentation

Mantoux – in vivo detection of specific cell immunity after exposition to antigen

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mantoux – in vivo detection of specific cell immunity after exposition to antigen • - burden of patient by antigen • - possible immunodeficiency of patients (anergy, risk of alergic reaction) • - memory cell after BCG vaccination, • - exposition to antigen = activation of MC • - interpretation, • - booster dose of antigen,

  2. Matoux skin test reading • application • positive negative 15 mm

  3. Measurement of induration • 0 – 15 mm – negative, not enought, vaccination, revaccination • 15 mm – OK, positive (after vaccination ?) • 15 and more – too much, possible activity

  4. in vitro detection of specific cell immunity after exposition to the antigen • whole blood of patient to 3 vials covered with specific antigen (M. tbc) • specific memory cells after disease are activated antigen is not given to patient in vivo • no risk of exposition of immunocompromised patients

  5. Test based on exposition of whole blood in 3 vials • antigens of M.tbc, - 1 vial - non specific mitogen – positive control - without antigen – negative control

  6. Vials are incubated 16 – 24 hrs at 37 dg C (sensibilised memory cells are exposed to specific antigen and start production of INTERFERONU GAMA – cytokín of CMI Centrifugation Harvest the plasma Storage

  7. To detect IF-g secreted after in vitro activation (antigen on the surface of vial + memory cells in the whole blood of pacient) of memory cells produced after the infection (by M.tbc) we use ELISA test

  8. Interpretation: comparison of concentration of IFg in the vial with -TB ag : negat.contr. Negat control – exclude the nonspecific reaction Pozit.control – demonstrate capacity to react (negat. in imunocompromised or annergic

  9. Mantoux: in vivo reactivity of memory cells produced after vaccination (BCG) and after disease (M.tbc) application of i.d. memory cells migrate to the place of injection inflamation with induration Quantiferon: in vitro memory cells after M.tbc infection present in the blood in vial + M.tbc antigen = release of IFg ELISA for detection comparison

  10. detection of cytokines • In vitro ( immunity of patient is not influenced, boostered) • specificity • sensitivity • not cross reacting antibodies PERSPECTIVES • detection of latent infection (any infection if the antigen is available) – in practice now only M.tbc CMI - scientifical purposes

  11. Other use Detection of latent infection • CMV – in transplantation, • tissue parasitoses – withou serological reaction, • systemic mycoses, • detection of CMI or memory cells – for identification of memory after vaccination, when antibodies are no more present, • detection of non responders

More Related